Bisdiamine inhibits extracellular matrix formation and cell proliferation of atrioventricular mesenchyme from developing chick heart valves

Michael Choy, Sharon L. Oltjen, Anita J. Moon, Margaret T. Armstrong, Peter B. Armstrong

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Abnormalities of the cushion tissues lead to atrioventricular septal defects (AVSD) and truncus arteriosus (TA). Bisdiamine exposure in the embryo frequently causes AVSD and TA in the newborn chick, mouse, or rat. We studied the effects of bisdiamine on mesenchymal cells grown in aggregate culture isolated from the developing atrioventricular valves of the stage-36 chick embryo. Fibronectin extracellular matrix formation and cell proliferation in the aggregates were assessed in various media. Chick serum stimulated the cells to produce an extracellular matrix and to divide, and the inclusion of bisdiamine inhibited both responses. If we isolated an extracellular matrix from a monolayer of mesenchymal cells and added the sonicated matrix to the medium containing serum and bisdiamine, the matrix incorporated into the aggregates and the cells entered the mitotic cycle. Our previous work established that cells need to attach to an intact extracellular matrix to begin cell division. Thus, we suggest that bisdiamine inhibits the normal formation of the extracellular matrix, leading to reduced cell proliferation, but it does not affect matrix-cell interaction. The lack of cushion growth in situ may be the cause of AVSD or TA.

Original languageEnglish (US)
Pages (from-to)148-155
Number of pages8
JournalTeratology
Volume59
Issue number3
StatePublished - 1999

Fingerprint

Heart Valves
Cell proliferation
Mesoderm
Extracellular Matrix
Truncus Arteriosus
Cells
Cell Proliferation
Defects
Fibronectins
Cell culture
Rats
Monolayers
Tissue
Chick Embryo
Serum
Cell Communication
Cell Division
Embryonic Structures
Growth
Atrioventricular Septal Defect

ASJC Scopus subject areas

  • Developmental Biology
  • Embryology
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Bisdiamine inhibits extracellular matrix formation and cell proliferation of atrioventricular mesenchyme from developing chick heart valves. / Choy, Michael; Oltjen, Sharon L.; Moon, Anita J.; Armstrong, Margaret T.; Armstrong, Peter B.

In: Teratology, Vol. 59, No. 3, 1999, p. 148-155.

Research output: Contribution to journalArticle

Choy, Michael ; Oltjen, Sharon L. ; Moon, Anita J. ; Armstrong, Margaret T. ; Armstrong, Peter B. / Bisdiamine inhibits extracellular matrix formation and cell proliferation of atrioventricular mesenchyme from developing chick heart valves. In: Teratology. 1999 ; Vol. 59, No. 3. pp. 148-155.
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