Biotyping of clinical isolates of Escherichia coli of animal origin, using the Analytab API 20E system.

F. Godbout-DeLasalle, Robert Higgins

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Using the Analytab (API 20E) Enterobacteriaceae system of biochemical identification, a total of 506 Escherichia coli isolates from different animal species were coded numerically or biotyped. Fifty-four different biotypes were identified, 11 accounting for 83.1% of the isolates examined. Three of these profiles accounted for 65.3% of the isolates and were found in almost all animal species. Some of the biotypes were found in only one animal species: six in cattle, five in horses, 15 in pigs, two in sheep, two in birds, one in dogs and one in a porpoise. Biotypes, as determined here, could not be related to a particular pathology and more work is needed to assess the extent and significance of this relative biotype specificity among animal species. The use of other, more sophisticated, typing systems, i.e. plasmid "fingerprinting", or restriction endonuclease analysis of chromosomal DNA, would have to be investigated.

Original languageEnglish (US)
Pages (from-to)418-421
Number of pages4
JournalCanadian journal of veterinary research = Revue canadienne de recherche veterinaire
Volume50
Issue number3
StatePublished - Jul 1 1986
Externally publishedYes

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biotypes
Escherichia coli
animals
Porpoises
Phocoenidae
restriction mapping
DNA Restriction Enzymes
Enterobacteriaceae
Horses
Birds
Sheep
plasmids
Plasmids
Swine
Dogs
Pathology
horses
sheep
swine
dogs

ASJC Scopus subject areas

  • veterinary(all)

Cite this

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abstract = "Using the Analytab (API 20E) Enterobacteriaceae system of biochemical identification, a total of 506 Escherichia coli isolates from different animal species were coded numerically or biotyped. Fifty-four different biotypes were identified, 11 accounting for 83.1{\%} of the isolates examined. Three of these profiles accounted for 65.3{\%} of the isolates and were found in almost all animal species. Some of the biotypes were found in only one animal species: six in cattle, five in horses, 15 in pigs, two in sheep, two in birds, one in dogs and one in a porpoise. Biotypes, as determined here, could not be related to a particular pathology and more work is needed to assess the extent and significance of this relative biotype specificity among animal species. The use of other, more sophisticated, typing systems, i.e. plasmid {"}fingerprinting{"}, or restriction endonuclease analysis of chromosomal DNA, would have to be investigated.",
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N2 - Using the Analytab (API 20E) Enterobacteriaceae system of biochemical identification, a total of 506 Escherichia coli isolates from different animal species were coded numerically or biotyped. Fifty-four different biotypes were identified, 11 accounting for 83.1% of the isolates examined. Three of these profiles accounted for 65.3% of the isolates and were found in almost all animal species. Some of the biotypes were found in only one animal species: six in cattle, five in horses, 15 in pigs, two in sheep, two in birds, one in dogs and one in a porpoise. Biotypes, as determined here, could not be related to a particular pathology and more work is needed to assess the extent and significance of this relative biotype specificity among animal species. The use of other, more sophisticated, typing systems, i.e. plasmid "fingerprinting", or restriction endonuclease analysis of chromosomal DNA, would have to be investigated.

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