Abstract
Primary biliary cirrhosis (PBC) is an autoimmune liver disease characterized by serum antimitochondrial antibody mainly reacting with inner lipoyl domain of E2 subunit of pyruvate dehydrogenase (PDC-E2). Lipoyl domain of PDC-E2 has sequence homologies with biotinylated site of biotin enzymes. This biotin-related protein and the C355.1 reactive antigen were surveyed by Western blotting in gallbladder bile samples from 27 patients with PBC and from 23 patients with other hepatobiliary disease. Biotin-related proteins of 70 and 72 kDa were detected in 89% of PBC samples, whereas it was infrequently detected in 48% of control samples (P < 0.01). C355.1 reactive antigen of 64 kDa was detected in 22% of PBC samples and 8.7% of control samples (P > 0.01). This biotin-related proteins of 70 and 72 kDa may refer to the pathogenesis of PBC.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 154-160 |
| Number of pages | 7 |
| Journal | Hepatology Research |
| Volume | 14 |
| Issue number | 2 |
| State | Published - Apr 1999 |
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Keywords
- Bile
- Biotin enzyme
- E2 subunit of pyruvate dehydrogenase
- Primary biliary cirrhosis
- Western blotting
ASJC Scopus subject areas
- Gastroenterology
Cite this
Biotin-related proteins of 70 and 72 kDa in gallbladder bile in primary biliary cirrhosis. / Sasaki, Motoko; Nakanuma, Yasuni; Gershwin, M. Eric; Demetris, Anthony J.; Wanless, Ian R.; Thung, Swang N.; Miyakawa, Hiroshi; Kage, Masayoshi; Mitsui, Takeshi.
In: Hepatology Research, Vol. 14, No. 2, 04.1999, p. 154-160.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Biotin-related proteins of 70 and 72 kDa in gallbladder bile in primary biliary cirrhosis
AU - Sasaki, Motoko
AU - Nakanuma, Yasuni
AU - Gershwin, M. Eric
AU - Demetris, Anthony J.
AU - Wanless, Ian R.
AU - Thung, Swang N.
AU - Miyakawa, Hiroshi
AU - Kage, Masayoshi
AU - Mitsui, Takeshi
PY - 1999/4
Y1 - 1999/4
N2 - Primary biliary cirrhosis (PBC) is an autoimmune liver disease characterized by serum antimitochondrial antibody mainly reacting with inner lipoyl domain of E2 subunit of pyruvate dehydrogenase (PDC-E2). Lipoyl domain of PDC-E2 has sequence homologies with biotinylated site of biotin enzymes. This biotin-related protein and the C355.1 reactive antigen were surveyed by Western blotting in gallbladder bile samples from 27 patients with PBC and from 23 patients with other hepatobiliary disease. Biotin-related proteins of 70 and 72 kDa were detected in 89% of PBC samples, whereas it was infrequently detected in 48% of control samples (P < 0.01). C355.1 reactive antigen of 64 kDa was detected in 22% of PBC samples and 8.7% of control samples (P > 0.01). This biotin-related proteins of 70 and 72 kDa may refer to the pathogenesis of PBC.
AB - Primary biliary cirrhosis (PBC) is an autoimmune liver disease characterized by serum antimitochondrial antibody mainly reacting with inner lipoyl domain of E2 subunit of pyruvate dehydrogenase (PDC-E2). Lipoyl domain of PDC-E2 has sequence homologies with biotinylated site of biotin enzymes. This biotin-related protein and the C355.1 reactive antigen were surveyed by Western blotting in gallbladder bile samples from 27 patients with PBC and from 23 patients with other hepatobiliary disease. Biotin-related proteins of 70 and 72 kDa were detected in 89% of PBC samples, whereas it was infrequently detected in 48% of control samples (P < 0.01). C355.1 reactive antigen of 64 kDa was detected in 22% of PBC samples and 8.7% of control samples (P > 0.01). This biotin-related proteins of 70 and 72 kDa may refer to the pathogenesis of PBC.
KW - Bile
KW - Biotin enzyme
KW - E2 subunit of pyruvate dehydrogenase
KW - Primary biliary cirrhosis
KW - Western blotting
UR - http://www.scopus.com/inward/record.url?scp=0032902637&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032902637&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0032902637
VL - 14
SP - 154
EP - 160
JO - International Hepatology Communications
JF - International Hepatology Communications
SN - 0928-4346
IS - 2
ER -