Biophotonic studies on lipid membranes using Oblique Incidence Reflectivity Difference (OI-RD) ellipsometry

R. Masina, X. D. Zhu, A. N. Parikh, R. Bruch, J. P. Landry

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

In this study we report new results on the changes in optical properties of glass surfaces induced during wet photolithography using Oblique Incidence Reflectivity Difference Ellipsometry (OI-RD). A novel wet UV-photolithographic method for patterning phospholipid bilayers into two-dimensional arrays of voids and patches on hydrophilic glass substrates is presented. Especially, this technique involves etching the glass substrate fused with lipid vesicle solution and subsequently illuminated with short-wavelength UV light through a photo mask thus creating voids in the irradiated regions. The effects of the chemical etching and subsequent UV irradiation on the surfaces of microscope glass slides are investigated using the OI-RD technique. In this study, we have observed that the UV irradiation after chemical etching further changes the properties of the surface, even in the absence of the lipid bilayer. As a result, irradiating the chemically etched surface before the UV photolithography step renders the whole surface homogenous. Furthermore, fluorescence recovery after photobleaching (FRAP) experiments have been conducted on such homogenized surfaces which reveal that the fluidic properties of the membranes are retained. The created patterns are suitable to study protein-DNA interactions in the lipid environment. Our long term goal is to utilize this technique as a new screening approach for testing drug interactions above and below the cell surface.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6095
DOIs
StatePublished - 2006
EventNanobiophotonics and Biomedical Applications III - San Jose, CA, United States
Duration: Jan 23 2006Jan 24 2006

Other

OtherNanobiophotonics and Biomedical Applications III
CountryUnited States
CitySan Jose, CA
Period1/23/061/24/06

Fingerprint

Ellipsometry
Etching
Glass
Photolithography
Lipids
Irradiation
Drug interactions
Photobleaching
Lipid bilayers
Phospholipids
Fluidics
Substrates
Membrane Lipids
Ultraviolet radiation
Masks
Screening
Microscopes
DNA
Optical properties
Fluorescence

Keywords

  • OI-RD ellipsometry
  • Phospholipid bilayers
  • Wet UV-photolithography

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Masina, R., Zhu, X. D., Parikh, A. N., Bruch, R., & Landry, J. P. (2006). Biophotonic studies on lipid membranes using Oblique Incidence Reflectivity Difference (OI-RD) ellipsometry. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 6095). [60950T] https://doi.org/10.1117/12.657478

Biophotonic studies on lipid membranes using Oblique Incidence Reflectivity Difference (OI-RD) ellipsometry. / Masina, R.; Zhu, X. D.; Parikh, A. N.; Bruch, R.; Landry, J. P.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6095 2006. 60950T.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Masina, R, Zhu, XD, Parikh, AN, Bruch, R & Landry, JP 2006, Biophotonic studies on lipid membranes using Oblique Incidence Reflectivity Difference (OI-RD) ellipsometry. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 6095, 60950T, Nanobiophotonics and Biomedical Applications III, San Jose, CA, United States, 1/23/06. https://doi.org/10.1117/12.657478
Masina R, Zhu XD, Parikh AN, Bruch R, Landry JP. Biophotonic studies on lipid membranes using Oblique Incidence Reflectivity Difference (OI-RD) ellipsometry. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6095. 2006. 60950T https://doi.org/10.1117/12.657478
Masina, R. ; Zhu, X. D. ; Parikh, A. N. ; Bruch, R. ; Landry, J. P. / Biophotonic studies on lipid membranes using Oblique Incidence Reflectivity Difference (OI-RD) ellipsometry. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6095 2006.
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