Biomechanical evaluation of suture-holding properties of native and tissue-engineered articular cartilage

G. D. DuRaine, Boaz Arzi, J. K. Lee, Cassandra A Lee, D. J. Responte, J. C. Hu, K. A. Athanasiou

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

The purpose of this study was to determine suture-holding properties of tissue-engineered neocartilage relative to native articular cartilage. To this end, suture pull-out strength was quantified for native articular cartilage and for neocartilages possessing various mechanical properties. Suture-holding properties were examined in vitro and in vivo. Neocartilage from bovine chondrocytes was engineered using two sets of exogenous stimuli, resulting in neotissue of different biochemical compositions. Compressive and tensile properties and glycosaminoglycan, collagen, and pyridinoline cross-link contents were assayed (study 1). Suture pull-out strength was compared between neocartilage constructs, and bovine and leporine native cartilage. Uniaxial pull-out test until failure was performed after passing 6-0 Vicryl through each tissue (study 2). Subsequently, neocartilage was implanted into a rabbit model to examine short-term suture-holding ability in vivo (study 3). Neocartilage glycosaminoglycan and collagen content per wet weight reached (Formula presented.) and (Formula presented.) , respectively. Tensile properties for neocartilage constructs reached (Formula presented.)  MPa for Young’s modulus and (Formula presented.)  MPa for ultimate tensile strength. Neocartilage reached (Formula presented.) 33 % of suture pull-out strength of native articular cartilage. Neocartilage cross-link content reached 50 % of native values, and suture pull-out strength correlated positively with cross-link content (Formula presented.). Neocartilage sutured into rabbit osteochondral defects was successfully maintained for 3 weeks. This study shows that pyridinoline cross-links in neocartilage may be vital in controlling suture pull-out strength. Neocartilage produced in vitro with one-third of native tissue pull-out strength appears sufficient for construct suturing and retention in vivo.

Original languageEnglish (US)
Pages (from-to)73-81
Number of pages9
JournalBiomechanics and Modeling in Mechanobiology
Volume14
Issue number1
DOIs
StatePublished - Jan 1 2015

Keywords

  • Articular cartilage
  • Self-assembling process
  • Suture pull-out
  • Tissue engineering

ASJC Scopus subject areas

  • Biotechnology
  • Mechanical Engineering
  • Modeling and Simulation
  • Medicine(all)

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