Biomarkers of chlorpyrifos exposure and effect in Egyptian cotton field workers

Fayssal M. Farahat, Corie A. Ellison, Matthew R. Bonner, Barbara P. Mcgarrigle, Alice L. Crane, Richard A. Fenske, Michael R. Lasarev, Diane S. Rohlman, W. Kent Anger, Pamela J Lein, James R. Olson

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Background: Chlorpyrifos (CPF), a widely used organophosphorus pesticide (OP), is metabolized to CPF-oxon, a potent cholinesterase (ChE) inhibitor, and trichloro-2-pyridinol (TCPy). Urinary TCPy is often used as a biomarker for CPF exposure, whereas blood ChE activity is considered an indicator of CPF toxicity. However, whether these biomarkers are dose related has not been studied extensively in populations with repeated daily OP exposures. Objective: We sought to determine the relationship between blood ChE and urinary TCPy during repeated occupational exposures to CPF. Methods: Daily urine samples and weekly blood samples were collected from pesticide workers (n = 38) in Menoufia Governorate, Egypt, before, during, and after 9-17 consecutive days of CPF application to cotton fields. We compared blood butyrylcholinesterase (BuChE) and acetylcholinesterase (AChE) activities with the respective urinary TCPy concentrations in each worker. Results: Average TCPy levels during the middle of a 1- to 2-week CPF application period were significantly higher in pesticide applicators (6,437 μg/g creatinine) than in technicians (184 μg/g) and engineers (157 μg/g), both of whom are involved in supervising the application process. We observed a statistically significant inverse correlation between urinary TCPy and blood BuChE and AChE activities. The no-effect level (or inflection point) of the exposure-effect relationships has an average urinary TCPy level of 114 μg/g creatinine for BuChE and 3,161 μg/g creatinine for AChE. Conclusions: Our findings demonstrate a dose-effect relationship between urinary TCPy and both plasma BuChE and red blood cell AChE in humans exposed occupationally to CPF. These findings will contribute to future risk assessment efforts for CPF exposure.

Original languageEnglish (US)
Pages (from-to)801-806
Number of pages6
JournalEnvironmental Health Perspectives
Volume119
Issue number6
DOIs
StatePublished - Jun 2011

Fingerprint

Chlorpyrifos
Health Personnel
Biomarkers
Butyrylcholinesterase
Acetylcholinesterase
Pesticides
Creatinine
Cholinesterases
Egypt
Cholinesterase Inhibitors
Occupational Exposure
Erythrocytes
Urine

Keywords

  • Acetylcholinesterase
  • Butyrylcholinesterase
  • Chlorpyrifos
  • Cholinesterase inhibition
  • Occupational exposure
  • Urinary TCPy

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Public Health, Environmental and Occupational Health

Cite this

Farahat, F. M., Ellison, C. A., Bonner, M. R., Mcgarrigle, B. P., Crane, A. L., Fenske, R. A., ... Olson, J. R. (2011). Biomarkers of chlorpyrifos exposure and effect in Egyptian cotton field workers. Environmental Health Perspectives, 119(6), 801-806. https://doi.org/10.1289/ehp.1002873

Biomarkers of chlorpyrifos exposure and effect in Egyptian cotton field workers. / Farahat, Fayssal M.; Ellison, Corie A.; Bonner, Matthew R.; Mcgarrigle, Barbara P.; Crane, Alice L.; Fenske, Richard A.; Lasarev, Michael R.; Rohlman, Diane S.; Anger, W. Kent; Lein, Pamela J; Olson, James R.

In: Environmental Health Perspectives, Vol. 119, No. 6, 06.2011, p. 801-806.

Research output: Contribution to journalArticle

Farahat, FM, Ellison, CA, Bonner, MR, Mcgarrigle, BP, Crane, AL, Fenske, RA, Lasarev, MR, Rohlman, DS, Anger, WK, Lein, PJ & Olson, JR 2011, 'Biomarkers of chlorpyrifos exposure and effect in Egyptian cotton field workers', Environmental Health Perspectives, vol. 119, no. 6, pp. 801-806. https://doi.org/10.1289/ehp.1002873
Farahat FM, Ellison CA, Bonner MR, Mcgarrigle BP, Crane AL, Fenske RA et al. Biomarkers of chlorpyrifos exposure and effect in Egyptian cotton field workers. Environmental Health Perspectives. 2011 Jun;119(6):801-806. https://doi.org/10.1289/ehp.1002873
Farahat, Fayssal M. ; Ellison, Corie A. ; Bonner, Matthew R. ; Mcgarrigle, Barbara P. ; Crane, Alice L. ; Fenske, Richard A. ; Lasarev, Michael R. ; Rohlman, Diane S. ; Anger, W. Kent ; Lein, Pamela J ; Olson, James R. / Biomarkers of chlorpyrifos exposure and effect in Egyptian cotton field workers. In: Environmental Health Perspectives. 2011 ; Vol. 119, No. 6. pp. 801-806.
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