Biological and biochemical characterization of a new isolate of feline sarcoma virus: Theilen-pedersen (TP1-FeSV)

Andrew Ziemiecki, Dagmar Hennig, Lance Gardner, Franz Josef Ferdinand, Robert R. Friis, Heinz Bauer, Niels C Pedersen, Loraine Johnson, Gordon H. Theilen

Research output: Contribution to journalArticle

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Abstract

A new feline sarcoma virus designated Theilen-Pedersen (TP1-FeSV) has been isolated from a spontaneous, slowly growing fibrosarcoma of a domestic short-haired 4-year-old castrated cat. The virus codes for a gag-onc fusion protein of 83,000 molecular weight phosphorylated in vivo at serine, threonine, and tyrosine residues. Cells transformed in vitro with TP1-FeSV exhibit five- to 10-fold elevated levels of phosphotyrosine over FeLV-infected cells. The gag-onc polyprotein has associated with it a tyrosyl protein kinase activity which in vitro results in autophosphorylation of the molecule at tyrosine residues. The fusion protein cannot be labeled metabolically with [3H]glucosamine and tunicamycin has no effect on the electrophoretic mobility of the in vivo [32P]orthophosphate-labeled fusion protein. The fusion protein, in common with the gag precursor Pr65gag, can be metabolically labeled with palmitic acid.

Original languageEnglish (US)
Pages (from-to)324-331
Number of pages8
JournalVirology
Volume138
Issue number2
DOIs
StatePublished - Oct 30 1984

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Feline Sarcoma Viruses
gag-onc Fusion Proteins
Tyrosine
Feline Leukemia Virus
gag Gene Products
Tunicamycin
Phosphotyrosine
Proteins
Palmitic Acid
Fibrosarcoma
Glucosamine
Threonine
Protein Kinases
Serine
Cats
Molecular Weight
Phosphates
Viruses
In Vitro Techniques

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Ziemiecki, A., Hennig, D., Gardner, L., Ferdinand, F. J., Friis, R. R., Bauer, H., ... Theilen, G. H. (1984). Biological and biochemical characterization of a new isolate of feline sarcoma virus: Theilen-pedersen (TP1-FeSV). Virology, 138(2), 324-331. https://doi.org/10.1016/0042-6822(84)90355-6

Biological and biochemical characterization of a new isolate of feline sarcoma virus : Theilen-pedersen (TP1-FeSV). / Ziemiecki, Andrew; Hennig, Dagmar; Gardner, Lance; Ferdinand, Franz Josef; Friis, Robert R.; Bauer, Heinz; Pedersen, Niels C; Johnson, Loraine; Theilen, Gordon H.

In: Virology, Vol. 138, No. 2, 30.10.1984, p. 324-331.

Research output: Contribution to journalArticle

Ziemiecki, A, Hennig, D, Gardner, L, Ferdinand, FJ, Friis, RR, Bauer, H, Pedersen, NC, Johnson, L & Theilen, GH 1984, 'Biological and biochemical characterization of a new isolate of feline sarcoma virus: Theilen-pedersen (TP1-FeSV)', Virology, vol. 138, no. 2, pp. 324-331. https://doi.org/10.1016/0042-6822(84)90355-6
Ziemiecki, Andrew ; Hennig, Dagmar ; Gardner, Lance ; Ferdinand, Franz Josef ; Friis, Robert R. ; Bauer, Heinz ; Pedersen, Niels C ; Johnson, Loraine ; Theilen, Gordon H. / Biological and biochemical characterization of a new isolate of feline sarcoma virus : Theilen-pedersen (TP1-FeSV). In: Virology. 1984 ; Vol. 138, No. 2. pp. 324-331.
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