Biological and biochemical characterization of a new isolate of feline sarcoma virus: Theilen-pedersen (TP1-FeSV)

Andrew Ziemiecki, Dagmar Hennig, Lance Gardner, Franz Josef Ferdinand, Robert R. Friis, Heinz Bauer, Niels C Pedersen, Loraine Johnson, Gordon H. Theilen

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Abstract

A new feline sarcoma virus designated Theilen-Pedersen (TP1-FeSV) has been isolated from a spontaneous, slowly growing fibrosarcoma of a domestic short-haired 4-year-old castrated cat. The virus codes for a gag-onc fusion protein of 83,000 molecular weight phosphorylated in vivo at serine, threonine, and tyrosine residues. Cells transformed in vitro with TP1-FeSV exhibit five- to 10-fold elevated levels of phosphotyrosine over FeLV-infected cells. The gag-onc polyprotein has associated with it a tyrosyl protein kinase activity which in vitro results in autophosphorylation of the molecule at tyrosine residues. The fusion protein cannot be labeled metabolically with [3H]glucosamine and tunicamycin has no effect on the electrophoretic mobility of the in vivo [32P]orthophosphate-labeled fusion protein. The fusion protein, in common with the gag precursor Pr65gag, can be metabolically labeled with palmitic acid.

Original languageEnglish (US)
Pages (from-to)324-331
Number of pages8
JournalVirology
Volume138
Issue number2
DOIs
StatePublished - Oct 30 1984

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ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Ziemiecki, A., Hennig, D., Gardner, L., Ferdinand, F. J., Friis, R. R., Bauer, H., Pedersen, N. C., Johnson, L., & Theilen, G. H. (1984). Biological and biochemical characterization of a new isolate of feline sarcoma virus: Theilen-pedersen (TP1-FeSV). Virology, 138(2), 324-331. https://doi.org/10.1016/0042-6822(84)90355-6