Abstract
Electrochemical nucleic acid sensors are promising tools for point-of-care diagnostic platforms with their facile integration with electronics and scalability. However, nucleic acid detection in complex biological fluids is challenging as biomolecules nonspecifically adsorb on the electrode surface and adversely affect the sensor performance by obscuring the transport of analytes and redox species to the electrode. We report that nanoporous gold (np-Au) electrodes, prepared by a microfabrication-compatible self-assembly process and functionalized with DNA probes, enabled detection of target DNA molecules (10-200 nM) in physiologically relevant complex media (bovine serum albumin and fetal bovine serum). In contrast, the sensor performance was compromised for planar gold electrodes in the same conditions. Hybridization efficiency decreased by 10% for np-Au with coarser pores revealing a pore-size dependence of sensor performance in biofouling conditions. This nanostructure-dependent functionality in complex media suggests that the pores with the optimal size and geometry act as sieves for blocking the biomolecules from inhibiting the surfaces within the porous volume while allowing the transport of nucleic acid analytes and redox molecules. (Figure Presented).
| Original language | English (US) |
|---|---|
| Pages (from-to) | 8618-8622 |
| Number of pages | 5 |
| Journal | Analytical Chemistry |
| Volume | 87 |
| Issue number | 17 |
| DOIs | |
| State | Published - Sep 1 2015 |
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ASJC Scopus subject areas
- Analytical Chemistry
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Biofouling-Resilient Nanoporous Gold Electrodes for DNA Sensing. / Daggumati, Pallavi; Matharu, Zimple; Wang, Ling; Seker, Erkin.
In: Analytical Chemistry, Vol. 87, No. 17, 01.09.2015, p. 8618-8622.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Biofouling-Resilient Nanoporous Gold Electrodes for DNA Sensing
AU - Daggumati, Pallavi
AU - Matharu, Zimple
AU - Wang, Ling
AU - Seker, Erkin
PY - 2015/9/1
Y1 - 2015/9/1
N2 - Electrochemical nucleic acid sensors are promising tools for point-of-care diagnostic platforms with their facile integration with electronics and scalability. However, nucleic acid detection in complex biological fluids is challenging as biomolecules nonspecifically adsorb on the electrode surface and adversely affect the sensor performance by obscuring the transport of analytes and redox species to the electrode. We report that nanoporous gold (np-Au) electrodes, prepared by a microfabrication-compatible self-assembly process and functionalized with DNA probes, enabled detection of target DNA molecules (10-200 nM) in physiologically relevant complex media (bovine serum albumin and fetal bovine serum). In contrast, the sensor performance was compromised for planar gold electrodes in the same conditions. Hybridization efficiency decreased by 10% for np-Au with coarser pores revealing a pore-size dependence of sensor performance in biofouling conditions. This nanostructure-dependent functionality in complex media suggests that the pores with the optimal size and geometry act as sieves for blocking the biomolecules from inhibiting the surfaces within the porous volume while allowing the transport of nucleic acid analytes and redox molecules. (Figure Presented).
AB - Electrochemical nucleic acid sensors are promising tools for point-of-care diagnostic platforms with their facile integration with electronics and scalability. However, nucleic acid detection in complex biological fluids is challenging as biomolecules nonspecifically adsorb on the electrode surface and adversely affect the sensor performance by obscuring the transport of analytes and redox species to the electrode. We report that nanoporous gold (np-Au) electrodes, prepared by a microfabrication-compatible self-assembly process and functionalized with DNA probes, enabled detection of target DNA molecules (10-200 nM) in physiologically relevant complex media (bovine serum albumin and fetal bovine serum). In contrast, the sensor performance was compromised for planar gold electrodes in the same conditions. Hybridization efficiency decreased by 10% for np-Au with coarser pores revealing a pore-size dependence of sensor performance in biofouling conditions. This nanostructure-dependent functionality in complex media suggests that the pores with the optimal size and geometry act as sieves for blocking the biomolecules from inhibiting the surfaces within the porous volume while allowing the transport of nucleic acid analytes and redox molecules. (Figure Presented).
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U2 - 10.1021/acs.analchem.5b02969
DO - 10.1021/acs.analchem.5b02969
M3 - Article
C2 - 26274576
AN - SCOPUS:84941010382
VL - 87
SP - 8618
EP - 8622
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 17
ER -