Biofouling-Resilient Nanoporous Gold Electrodes for DNA Sensing

Pallavi Daggumati, Zimple Matharu, Ling Wang, Erkin Seker

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Electrochemical nucleic acid sensors are promising tools for point-of-care diagnostic platforms with their facile integration with electronics and scalability. However, nucleic acid detection in complex biological fluids is challenging as biomolecules nonspecifically adsorb on the electrode surface and adversely affect the sensor performance by obscuring the transport of analytes and redox species to the electrode. We report that nanoporous gold (np-Au) electrodes, prepared by a microfabrication-compatible self-assembly process and functionalized with DNA probes, enabled detection of target DNA molecules (10-200 nM) in physiologically relevant complex media (bovine serum albumin and fetal bovine serum). In contrast, the sensor performance was compromised for planar gold electrodes in the same conditions. Hybridization efficiency decreased by 10% for np-Au with coarser pores revealing a pore-size dependence of sensor performance in biofouling conditions. This nanostructure-dependent functionality in complex media suggests that the pores with the optimal size and geometry act as sieves for blocking the biomolecules from inhibiting the surfaces within the porous volume while allowing the transport of nucleic acid analytes and redox molecules. (Figure Presented).

Original languageEnglish (US)
Pages (from-to)8618-8622
Number of pages5
JournalAnalytical Chemistry
Volume87
Issue number17
DOIs
StatePublished - Sep 1 2015

Fingerprint

Biofouling
Gold
Nucleic Acids
Electrodes
DNA
Sensors
Biomolecules
Molecules
Sieves
Microfabrication
DNA Probes
Bovine Serum Albumin
Self assembly
Pore size
Scalability
Nanostructures
Electronic equipment
Fluids
Geometry
Oxidation-Reduction

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Biofouling-Resilient Nanoporous Gold Electrodes for DNA Sensing. / Daggumati, Pallavi; Matharu, Zimple; Wang, Ling; Seker, Erkin.

In: Analytical Chemistry, Vol. 87, No. 17, 01.09.2015, p. 8618-8622.

Research output: Contribution to journalArticle

Daggumati, Pallavi ; Matharu, Zimple ; Wang, Ling ; Seker, Erkin. / Biofouling-Resilient Nanoporous Gold Electrodes for DNA Sensing. In: Analytical Chemistry. 2015 ; Vol. 87, No. 17. pp. 8618-8622.
@article{a8c380315a6446cb93bca0697b535b9b,
title = "Biofouling-Resilient Nanoporous Gold Electrodes for DNA Sensing",
abstract = "Electrochemical nucleic acid sensors are promising tools for point-of-care diagnostic platforms with their facile integration with electronics and scalability. However, nucleic acid detection in complex biological fluids is challenging as biomolecules nonspecifically adsorb on the electrode surface and adversely affect the sensor performance by obscuring the transport of analytes and redox species to the electrode. We report that nanoporous gold (np-Au) electrodes, prepared by a microfabrication-compatible self-assembly process and functionalized with DNA probes, enabled detection of target DNA molecules (10-200 nM) in physiologically relevant complex media (bovine serum albumin and fetal bovine serum). In contrast, the sensor performance was compromised for planar gold electrodes in the same conditions. Hybridization efficiency decreased by 10{\%} for np-Au with coarser pores revealing a pore-size dependence of sensor performance in biofouling conditions. This nanostructure-dependent functionality in complex media suggests that the pores with the optimal size and geometry act as sieves for blocking the biomolecules from inhibiting the surfaces within the porous volume while allowing the transport of nucleic acid analytes and redox molecules. (Figure Presented).",
author = "Pallavi Daggumati and Zimple Matharu and Ling Wang and Erkin Seker",
year = "2015",
month = "9",
day = "1",
doi = "10.1021/acs.analchem.5b02969",
language = "English (US)",
volume = "87",
pages = "8618--8622",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "17",

}

TY - JOUR

T1 - Biofouling-Resilient Nanoporous Gold Electrodes for DNA Sensing

AU - Daggumati, Pallavi

AU - Matharu, Zimple

AU - Wang, Ling

AU - Seker, Erkin

PY - 2015/9/1

Y1 - 2015/9/1

N2 - Electrochemical nucleic acid sensors are promising tools for point-of-care diagnostic platforms with their facile integration with electronics and scalability. However, nucleic acid detection in complex biological fluids is challenging as biomolecules nonspecifically adsorb on the electrode surface and adversely affect the sensor performance by obscuring the transport of analytes and redox species to the electrode. We report that nanoporous gold (np-Au) electrodes, prepared by a microfabrication-compatible self-assembly process and functionalized with DNA probes, enabled detection of target DNA molecules (10-200 nM) in physiologically relevant complex media (bovine serum albumin and fetal bovine serum). In contrast, the sensor performance was compromised for planar gold electrodes in the same conditions. Hybridization efficiency decreased by 10% for np-Au with coarser pores revealing a pore-size dependence of sensor performance in biofouling conditions. This nanostructure-dependent functionality in complex media suggests that the pores with the optimal size and geometry act as sieves for blocking the biomolecules from inhibiting the surfaces within the porous volume while allowing the transport of nucleic acid analytes and redox molecules. (Figure Presented).

AB - Electrochemical nucleic acid sensors are promising tools for point-of-care diagnostic platforms with their facile integration with electronics and scalability. However, nucleic acid detection in complex biological fluids is challenging as biomolecules nonspecifically adsorb on the electrode surface and adversely affect the sensor performance by obscuring the transport of analytes and redox species to the electrode. We report that nanoporous gold (np-Au) electrodes, prepared by a microfabrication-compatible self-assembly process and functionalized with DNA probes, enabled detection of target DNA molecules (10-200 nM) in physiologically relevant complex media (bovine serum albumin and fetal bovine serum). In contrast, the sensor performance was compromised for planar gold electrodes in the same conditions. Hybridization efficiency decreased by 10% for np-Au with coarser pores revealing a pore-size dependence of sensor performance in biofouling conditions. This nanostructure-dependent functionality in complex media suggests that the pores with the optimal size and geometry act as sieves for blocking the biomolecules from inhibiting the surfaces within the porous volume while allowing the transport of nucleic acid analytes and redox molecules. (Figure Presented).

UR - http://www.scopus.com/inward/record.url?scp=84941010382&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84941010382&partnerID=8YFLogxK

U2 - 10.1021/acs.analchem.5b02969

DO - 10.1021/acs.analchem.5b02969

M3 - Article

C2 - 26274576

AN - SCOPUS:84941010382

VL - 87

SP - 8618

EP - 8622

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 17

ER -