Abstract
Francisella noatunensis subsp. orientalis (Fno) is an emergent fish pathogen in both marine and fresh water environments. The bacterium is suspected to persist in the environment even without the presence of a suitable fish host. In the present study, the influence of different abiotic factors such as salinity and temperature were used to study the biofilm formation of different isolates of Fno including intracellular growth loci C (iglC) and pathogenicity determinant protein A (pdpA) knockout strains. Finally, we compared the susceptibility of planktonic and biofilm to three disinfectants used in the aquaculture and ornamental fish industry, namely Virkon®, bleach and hydrogen peroxide. The data indicates that Fno is capable of producing biofilms within 24 h where both salinity as well as temperature plays a role in the growth and biofilm formation of Fno. Mutations in the iglC or pdpA, both known virulence factors, do not appear to affect the capacity of Fno to produce biofilms, and the minimum inhibitory concentration, and minimum biocidal concentration for the three disinfectants were lower than the minimum biofilm eradication concentration values. This information needs to be taken into account if trying to eradicate the pathogen from aquaculture facilities or aquariums.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 313-317 |
| Number of pages | 5 |
| Journal | Veterinary Microbiology |
| Volume | 181 |
| Issue number | 3-4 |
| DOIs | |
| State | Published - Dec 31 2015 |
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Keywords
- Biofilm
- Francisella noatunensis
- Francisellosis
ASJC Scopus subject areas
- Microbiology
- veterinary(all)
Cite this
Biofilm formation of Francisella noatunensis subsp. orientalis. / Soto Martinez, Esteban; Halliday-Simmonds, Iona; Francis, Stewart; Kearney, Michael T.; Hansen, John D.
In: Veterinary Microbiology, Vol. 181, No. 3-4, 31.12.2015, p. 313-317.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Biofilm formation of Francisella noatunensis subsp. orientalis
AU - Soto Martinez, Esteban
AU - Halliday-Simmonds, Iona
AU - Francis, Stewart
AU - Kearney, Michael T.
AU - Hansen, John D.
PY - 2015/12/31
Y1 - 2015/12/31
N2 - Francisella noatunensis subsp. orientalis (Fno) is an emergent fish pathogen in both marine and fresh water environments. The bacterium is suspected to persist in the environment even without the presence of a suitable fish host. In the present study, the influence of different abiotic factors such as salinity and temperature were used to study the biofilm formation of different isolates of Fno including intracellular growth loci C (iglC) and pathogenicity determinant protein A (pdpA) knockout strains. Finally, we compared the susceptibility of planktonic and biofilm to three disinfectants used in the aquaculture and ornamental fish industry, namely Virkon®, bleach and hydrogen peroxide. The data indicates that Fno is capable of producing biofilms within 24 h where both salinity as well as temperature plays a role in the growth and biofilm formation of Fno. Mutations in the iglC or pdpA, both known virulence factors, do not appear to affect the capacity of Fno to produce biofilms, and the minimum inhibitory concentration, and minimum biocidal concentration for the three disinfectants were lower than the minimum biofilm eradication concentration values. This information needs to be taken into account if trying to eradicate the pathogen from aquaculture facilities or aquariums.
AB - Francisella noatunensis subsp. orientalis (Fno) is an emergent fish pathogen in both marine and fresh water environments. The bacterium is suspected to persist in the environment even without the presence of a suitable fish host. In the present study, the influence of different abiotic factors such as salinity and temperature were used to study the biofilm formation of different isolates of Fno including intracellular growth loci C (iglC) and pathogenicity determinant protein A (pdpA) knockout strains. Finally, we compared the susceptibility of planktonic and biofilm to three disinfectants used in the aquaculture and ornamental fish industry, namely Virkon®, bleach and hydrogen peroxide. The data indicates that Fno is capable of producing biofilms within 24 h where both salinity as well as temperature plays a role in the growth and biofilm formation of Fno. Mutations in the iglC or pdpA, both known virulence factors, do not appear to affect the capacity of Fno to produce biofilms, and the minimum inhibitory concentration, and minimum biocidal concentration for the three disinfectants were lower than the minimum biofilm eradication concentration values. This information needs to be taken into account if trying to eradicate the pathogen from aquaculture facilities or aquariums.
KW - Biofilm
KW - Francisella noatunensis
KW - Francisellosis
UR - http://www.scopus.com/inward/record.url?scp=84951103201&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84951103201&partnerID=8YFLogxK
U2 - 10.1016/j.vetmic.2015.10.007
DO - 10.1016/j.vetmic.2015.10.007
M3 - Article
VL - 181
SP - 313
EP - 317
JO - Veterinary Microbiology
JF - Veterinary Microbiology
SN - 0378-1135
IS - 3-4
ER -