Biofilm formation of Francisella noatunensis subsp. orientalis

Esteban Soto Martinez, Iona Halliday-Simmonds, Stewart Francis, Michael T. Kearney, John D. Hansen

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Francisella noatunensis subsp. orientalis (Fno) is an emergent fish pathogen in both marine and fresh water environments. The bacterium is suspected to persist in the environment even without the presence of a suitable fish host. In the present study, the influence of different abiotic factors such as salinity and temperature were used to study the biofilm formation of different isolates of Fno including intracellular growth loci C (iglC) and pathogenicity determinant protein A (pdpA) knockout strains. Finally, we compared the susceptibility of planktonic and biofilm to three disinfectants used in the aquaculture and ornamental fish industry, namely Virkon®, bleach and hydrogen peroxide. The data indicates that Fno is capable of producing biofilms within 24 h where both salinity as well as temperature plays a role in the growth and biofilm formation of Fno. Mutations in the iglC or pdpA, both known virulence factors, do not appear to affect the capacity of Fno to produce biofilms, and the minimum inhibitory concentration, and minimum biocidal concentration for the three disinfectants were lower than the minimum biofilm eradication concentration values. This information needs to be taken into account if trying to eradicate the pathogen from aquaculture facilities or aquariums.

Original languageEnglish (US)
Pages (from-to)313-317
Number of pages5
JournalVeterinary Microbiology
Volume181
Issue number3-4
DOIs
StatePublished - Dec 31 2015

Fingerprint

Francisella
Biofilms
biofilm
Aquaculture
Fishes
Disinfectants
disinfectants
Salinity
Staphylococcal Protein A
Virulence
aquaculture
pathogenicity
Growth
salinity
ornamental fish
fish industry
loci
Temperature
pathogens
Microbial Sensitivity Tests

Keywords

  • Biofilm
  • Francisella noatunensis
  • Francisellosis

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

Cite this

Soto Martinez, E., Halliday-Simmonds, I., Francis, S., Kearney, M. T., & Hansen, J. D. (2015). Biofilm formation of Francisella noatunensis subsp. orientalis. Veterinary Microbiology, 181(3-4), 313-317. https://doi.org/10.1016/j.vetmic.2015.10.007

Biofilm formation of Francisella noatunensis subsp. orientalis. / Soto Martinez, Esteban; Halliday-Simmonds, Iona; Francis, Stewart; Kearney, Michael T.; Hansen, John D.

In: Veterinary Microbiology, Vol. 181, No. 3-4, 31.12.2015, p. 313-317.

Research output: Contribution to journalArticle

Soto Martinez, E, Halliday-Simmonds, I, Francis, S, Kearney, MT & Hansen, JD 2015, 'Biofilm formation of Francisella noatunensis subsp. orientalis', Veterinary Microbiology, vol. 181, no. 3-4, pp. 313-317. https://doi.org/10.1016/j.vetmic.2015.10.007
Soto Martinez, Esteban ; Halliday-Simmonds, Iona ; Francis, Stewart ; Kearney, Michael T. ; Hansen, John D. / Biofilm formation of Francisella noatunensis subsp. orientalis. In: Veterinary Microbiology. 2015 ; Vol. 181, No. 3-4. pp. 313-317.
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