Biochemistry Retrovirus insertion into herpesvirus in vitro and in vivo

Robert Isfort, Dan Jones, Rhonda Kost, Richard Witter, Hsing-Jien Kung

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

Retroviruses and herpesviruses are naturally occurring pathogens of humans and animals. Coinfection of the same host with both these viruses is common. We report here that a retrovirus can integrate directly into a herpesvirus genome. Specifically, we demonstrate insertion of a nonacute retrovirus, reticuloendotheliosis virus (REV), into a herpesvirus, Marek disease virus (MDV). Both viruses are capable of inducing T lymphomas in chickens and often coexist in the same animal. REV DNA integration into MDV occurred in a recently attenuated strain of MDV and in a short-term coinfection experiment in vitro. We also provide suggestive evidence that REV has inserted into pathogenic strains of MDV in the past. Sequences homologous to the REV long terminal repeat are found in oncogenic MDV but not in nononcogenic strains. These results raise the possibility that retroviral information may be transmitted by herpesvirus and that herpesvirus expression can be modulated by retroviral elements. In addition, retrovirus may provide a useful tool to characterize herpesviral function by insertional mutagenesis.

Original languageEnglish (US)
Pages (from-to)991-995
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number3
StatePublished - Feb 1 1992
Externally publishedYes

Fingerprint

Herpesviridae
Retroviridae
Marek Disease
Biochemistry
Reticuloendotheliosis virus
Viruses
Coinfection
Virus Integration
Terminal Repeat Sequences
Insertional Mutagenesis
Sequence Homology
In Vitro Techniques
Chickens
Lymphoma
Genome
DNA

Keywords

  • Long terminal repeat
  • Marek disease virus
  • Reticuloendotheliosis virus
  • T lymphoma

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Biochemistry Retrovirus insertion into herpesvirus in vitro and in vivo. / Isfort, Robert; Jones, Dan; Kost, Rhonda; Witter, Richard; Kung, Hsing-Jien.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, No. 3, 01.02.1992, p. 991-995.

Research output: Contribution to journalArticle

@article{af07ebc281fb4be8ac196ac27b8e1ab1,
title = "Biochemistry Retrovirus insertion into herpesvirus in vitro and in vivo",
abstract = "Retroviruses and herpesviruses are naturally occurring pathogens of humans and animals. Coinfection of the same host with both these viruses is common. We report here that a retrovirus can integrate directly into a herpesvirus genome. Specifically, we demonstrate insertion of a nonacute retrovirus, reticuloendotheliosis virus (REV), into a herpesvirus, Marek disease virus (MDV). Both viruses are capable of inducing T lymphomas in chickens and often coexist in the same animal. REV DNA integration into MDV occurred in a recently attenuated strain of MDV and in a short-term coinfection experiment in vitro. We also provide suggestive evidence that REV has inserted into pathogenic strains of MDV in the past. Sequences homologous to the REV long terminal repeat are found in oncogenic MDV but not in nononcogenic strains. These results raise the possibility that retroviral information may be transmitted by herpesvirus and that herpesvirus expression can be modulated by retroviral elements. In addition, retrovirus may provide a useful tool to characterize herpesviral function by insertional mutagenesis.",
keywords = "Long terminal repeat, Marek disease virus, Reticuloendotheliosis virus, T lymphoma",
author = "Robert Isfort and Dan Jones and Rhonda Kost and Richard Witter and Hsing-Jien Kung",
year = "1992",
month = "2",
day = "1",
language = "English (US)",
volume = "89",
pages = "991--995",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "3",

}

TY - JOUR

T1 - Biochemistry Retrovirus insertion into herpesvirus in vitro and in vivo

AU - Isfort, Robert

AU - Jones, Dan

AU - Kost, Rhonda

AU - Witter, Richard

AU - Kung, Hsing-Jien

PY - 1992/2/1

Y1 - 1992/2/1

N2 - Retroviruses and herpesviruses are naturally occurring pathogens of humans and animals. Coinfection of the same host with both these viruses is common. We report here that a retrovirus can integrate directly into a herpesvirus genome. Specifically, we demonstrate insertion of a nonacute retrovirus, reticuloendotheliosis virus (REV), into a herpesvirus, Marek disease virus (MDV). Both viruses are capable of inducing T lymphomas in chickens and often coexist in the same animal. REV DNA integration into MDV occurred in a recently attenuated strain of MDV and in a short-term coinfection experiment in vitro. We also provide suggestive evidence that REV has inserted into pathogenic strains of MDV in the past. Sequences homologous to the REV long terminal repeat are found in oncogenic MDV but not in nononcogenic strains. These results raise the possibility that retroviral information may be transmitted by herpesvirus and that herpesvirus expression can be modulated by retroviral elements. In addition, retrovirus may provide a useful tool to characterize herpesviral function by insertional mutagenesis.

AB - Retroviruses and herpesviruses are naturally occurring pathogens of humans and animals. Coinfection of the same host with both these viruses is common. We report here that a retrovirus can integrate directly into a herpesvirus genome. Specifically, we demonstrate insertion of a nonacute retrovirus, reticuloendotheliosis virus (REV), into a herpesvirus, Marek disease virus (MDV). Both viruses are capable of inducing T lymphomas in chickens and often coexist in the same animal. REV DNA integration into MDV occurred in a recently attenuated strain of MDV and in a short-term coinfection experiment in vitro. We also provide suggestive evidence that REV has inserted into pathogenic strains of MDV in the past. Sequences homologous to the REV long terminal repeat are found in oncogenic MDV but not in nononcogenic strains. These results raise the possibility that retroviral information may be transmitted by herpesvirus and that herpesvirus expression can be modulated by retroviral elements. In addition, retrovirus may provide a useful tool to characterize herpesviral function by insertional mutagenesis.

KW - Long terminal repeat

KW - Marek disease virus

KW - Reticuloendotheliosis virus

KW - T lymphoma

UR - http://www.scopus.com/inward/record.url?scp=0026547533&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026547533&partnerID=8YFLogxK

M3 - Article

VL - 89

SP - 991

EP - 995

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 3

ER -