Biochemistry of eukaryotic homologous recombination

Wolf Dietrich Heyer

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The biochemistry of eukaryotic homologous recombination caught fire with the discovery that Rad51 is the eukaryotic homolog of the bacterial RecA and T4 UvsX proteins; and this field is still hot. The core reaction of homologous recombination, homology search and DNA strand invasion, along with the proteins catalyzing it, are conserved throughout evolution in principle. However, the increased complexity of eukaryotic genomes and the diversity of eukaryotic cell biology pose additional challenges to the recombination machinery. It is not surprising that this increase in complexity coincided with the evolution of new recombination proteins and novel support pathways, as well as changes in the properties of those eukaryotic recombination proteins that are evidently conserved in evolution. In humans, defects in homologous recombination lead to increased cancer predisposition, underlining the importance of this pathway for genomic stability and tumor suppression. This review will focus on the mechanisms of homologous recombination in eukaryotes as elucidated by the biochemical analysis of yeast and human proteins.

Original languageEnglish (US)
Pages (from-to)251-283
Number of pages33
JournalTopics in Current Genetics
Volume17
DOIs
StatePublished - 2007

Fingerprint

Homologous Recombination
Biochemistry
Genetic Recombination
Proteins
Fungal Proteins
Genomic Instability
Eukaryotic Cells
Eukaryota
Cell Biology
Neoplasms
Genome
DNA

ASJC Scopus subject areas

  • Genetics
  • Cell Biology
  • Molecular Biology
  • Developmental Biology

Cite this

Biochemistry of eukaryotic homologous recombination. / Heyer, Wolf Dietrich.

In: Topics in Current Genetics, Vol. 17, 2007, p. 251-283.

Research output: Contribution to journalArticle

Heyer, Wolf Dietrich. / Biochemistry of eukaryotic homologous recombination. In: Topics in Current Genetics. 2007 ; Vol. 17. pp. 251-283.
@article{42a03cc8f717414197e1c23abd3c9260,
title = "Biochemistry of eukaryotic homologous recombination",
abstract = "The biochemistry of eukaryotic homologous recombination caught fire with the discovery that Rad51 is the eukaryotic homolog of the bacterial RecA and T4 UvsX proteins; and this field is still hot. The core reaction of homologous recombination, homology search and DNA strand invasion, along with the proteins catalyzing it, are conserved throughout evolution in principle. However, the increased complexity of eukaryotic genomes and the diversity of eukaryotic cell biology pose additional challenges to the recombination machinery. It is not surprising that this increase in complexity coincided with the evolution of new recombination proteins and novel support pathways, as well as changes in the properties of those eukaryotic recombination proteins that are evidently conserved in evolution. In humans, defects in homologous recombination lead to increased cancer predisposition, underlining the importance of this pathway for genomic stability and tumor suppression. This review will focus on the mechanisms of homologous recombination in eukaryotes as elucidated by the biochemical analysis of yeast and human proteins.",
author = "Heyer, {Wolf Dietrich}",
year = "2007",
doi = "10.1007/4735_2006_0209",
language = "English (US)",
volume = "17",
pages = "251--283",
journal = "Topics in Current Genetics",
issn = "1610-2096",
publisher = "Springer Verlag",

}

TY - JOUR

T1 - Biochemistry of eukaryotic homologous recombination

AU - Heyer, Wolf Dietrich

PY - 2007

Y1 - 2007

N2 - The biochemistry of eukaryotic homologous recombination caught fire with the discovery that Rad51 is the eukaryotic homolog of the bacterial RecA and T4 UvsX proteins; and this field is still hot. The core reaction of homologous recombination, homology search and DNA strand invasion, along with the proteins catalyzing it, are conserved throughout evolution in principle. However, the increased complexity of eukaryotic genomes and the diversity of eukaryotic cell biology pose additional challenges to the recombination machinery. It is not surprising that this increase in complexity coincided with the evolution of new recombination proteins and novel support pathways, as well as changes in the properties of those eukaryotic recombination proteins that are evidently conserved in evolution. In humans, defects in homologous recombination lead to increased cancer predisposition, underlining the importance of this pathway for genomic stability and tumor suppression. This review will focus on the mechanisms of homologous recombination in eukaryotes as elucidated by the biochemical analysis of yeast and human proteins.

AB - The biochemistry of eukaryotic homologous recombination caught fire with the discovery that Rad51 is the eukaryotic homolog of the bacterial RecA and T4 UvsX proteins; and this field is still hot. The core reaction of homologous recombination, homology search and DNA strand invasion, along with the proteins catalyzing it, are conserved throughout evolution in principle. However, the increased complexity of eukaryotic genomes and the diversity of eukaryotic cell biology pose additional challenges to the recombination machinery. It is not surprising that this increase in complexity coincided with the evolution of new recombination proteins and novel support pathways, as well as changes in the properties of those eukaryotic recombination proteins that are evidently conserved in evolution. In humans, defects in homologous recombination lead to increased cancer predisposition, underlining the importance of this pathway for genomic stability and tumor suppression. This review will focus on the mechanisms of homologous recombination in eukaryotes as elucidated by the biochemical analysis of yeast and human proteins.

UR - http://www.scopus.com/inward/record.url?scp=34247507350&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34247507350&partnerID=8YFLogxK

U2 - 10.1007/4735_2006_0209

DO - 10.1007/4735_2006_0209

M3 - Article

VL - 17

SP - 251

EP - 283

JO - Topics in Current Genetics

JF - Topics in Current Genetics

SN - 1610-2096

ER -