Biochemical effects of intratracheal instillation of cadmium chloride on rat lung

Carroll E Cross, Stanley T. Omaye, Donald C. Rifas, Glen K. Hasegawa, Krishna A. Reddy

Research output: Contribution to journalArticle

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Abstract

Selected biochemical parameters in rat lung tissue were examined after intratracheal instillation of 0.5 μmole/kg of cadmium chloride (CdCl2), which produces levels of approximately 10 μg Cd/g of lung wet weight 1 hr following instillation. Lungs were examined 2 hr, 1 day, 3 days and 7 days after CdCl2 instillation and compared with matched controls receiving saline instillations. Cytosolic lysosomal enzymes, Superoxide dismutase (SOD), catalase, and glutathione (GSH) peroxidase-associated enzymes increased significantly 24 hr after CdCl2 insult. Peak increases of enzyme activities occurred about 3 days after CdCl2 instillation. Levels of lung nonprotein sulfhydryl (NPSH) groups, thiobarbituric acid (TBA)-reactive products, protein and DNA increased after CdCl2 instillation in a similar manner. By 7 days most measured biochemical parameters either remained at the peak 3-day values or decreased toward normal levels. The biochemical changes are consistent with known reported CdCl2-induced edema and inflammation accompanied by phagocyte recruitment into lung tissue and reparative proliferation of lung cell types.

Original languageEnglish (US)
Pages (from-to)381-388
Number of pages8
JournalBiochemical Pharmacology
Volume28
Issue number3
DOIs
StatePublished - 1979

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Cadmium Chloride
Rats
Lung
Enzymes
Tissue
Enzyme activity
Pulmonary Edema
Phagocytes
Glutathione Peroxidase
Catalase
Peroxidase
Superoxide Dismutase
Glutathione
Edema
Cell Proliferation
Inflammation
Weights and Measures
DNA
Proteins

ASJC Scopus subject areas

  • Pharmacology

Cite this

Biochemical effects of intratracheal instillation of cadmium chloride on rat lung. / Cross, Carroll E; Omaye, Stanley T.; Rifas, Donald C.; Hasegawa, Glen K.; Reddy, Krishna A.

In: Biochemical Pharmacology, Vol. 28, No. 3, 1979, p. 381-388.

Research output: Contribution to journalArticle

Cross, Carroll E ; Omaye, Stanley T. ; Rifas, Donald C. ; Hasegawa, Glen K. ; Reddy, Krishna A. / Biochemical effects of intratracheal instillation of cadmium chloride on rat lung. In: Biochemical Pharmacology. 1979 ; Vol. 28, No. 3. pp. 381-388.
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