Abstract
Selected biochemical parameters in rat lung tissue were examined after intratracheal instillation of 0.5 μmole/kg of cadmium chloride (CdCl2), which produces levels of approximately 10 μg Cd/g of lung wet weight 1 hr following instillation. Lungs were examined 2 hr, 1 day, 3 days and 7 days after CdCl2 instillation and compared with matched controls receiving saline instillations. Cytosolic lysosomal enzymes, Superoxide dismutase (SOD), catalase, and glutathione (GSH) peroxidase-associated enzymes increased significantly 24 hr after CdCl2 insult. Peak increases of enzyme activities occurred about 3 days after CdCl2 instillation. Levels of lung nonprotein sulfhydryl (NPSH) groups, thiobarbituric acid (TBA)-reactive products, protein and DNA increased after CdCl2 instillation in a similar manner. By 7 days most measured biochemical parameters either remained at the peak 3-day values or decreased toward normal levels. The biochemical changes are consistent with known reported CdCl2-induced edema and inflammation accompanied by phagocyte recruitment into lung tissue and reparative proliferation of lung cell types.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 381-388 |
| Number of pages | 8 |
| Journal | Biochemical Pharmacology |
| Volume | 28 |
| Issue number | 3 |
| DOIs | |
| State | Published - 1979 |
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ASJC Scopus subject areas
- Pharmacology
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Biochemical effects of intratracheal instillation of cadmium chloride on rat lung. / Cross, Carroll E; Omaye, Stanley T.; Rifas, Donald C.; Hasegawa, Glen K.; Reddy, Krishna A.
In: Biochemical Pharmacology, Vol. 28, No. 3, 1979, p. 381-388.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Biochemical effects of intratracheal instillation of cadmium chloride on rat lung
AU - Cross, Carroll E
AU - Omaye, Stanley T.
AU - Rifas, Donald C.
AU - Hasegawa, Glen K.
AU - Reddy, Krishna A.
PY - 1979
Y1 - 1979
N2 - Selected biochemical parameters in rat lung tissue were examined after intratracheal instillation of 0.5 μmole/kg of cadmium chloride (CdCl2), which produces levels of approximately 10 μg Cd/g of lung wet weight 1 hr following instillation. Lungs were examined 2 hr, 1 day, 3 days and 7 days after CdCl2 instillation and compared with matched controls receiving saline instillations. Cytosolic lysosomal enzymes, Superoxide dismutase (SOD), catalase, and glutathione (GSH) peroxidase-associated enzymes increased significantly 24 hr after CdCl2 insult. Peak increases of enzyme activities occurred about 3 days after CdCl2 instillation. Levels of lung nonprotein sulfhydryl (NPSH) groups, thiobarbituric acid (TBA)-reactive products, protein and DNA increased after CdCl2 instillation in a similar manner. By 7 days most measured biochemical parameters either remained at the peak 3-day values or decreased toward normal levels. The biochemical changes are consistent with known reported CdCl2-induced edema and inflammation accompanied by phagocyte recruitment into lung tissue and reparative proliferation of lung cell types.
AB - Selected biochemical parameters in rat lung tissue were examined after intratracheal instillation of 0.5 μmole/kg of cadmium chloride (CdCl2), which produces levels of approximately 10 μg Cd/g of lung wet weight 1 hr following instillation. Lungs were examined 2 hr, 1 day, 3 days and 7 days after CdCl2 instillation and compared with matched controls receiving saline instillations. Cytosolic lysosomal enzymes, Superoxide dismutase (SOD), catalase, and glutathione (GSH) peroxidase-associated enzymes increased significantly 24 hr after CdCl2 insult. Peak increases of enzyme activities occurred about 3 days after CdCl2 instillation. Levels of lung nonprotein sulfhydryl (NPSH) groups, thiobarbituric acid (TBA)-reactive products, protein and DNA increased after CdCl2 instillation in a similar manner. By 7 days most measured biochemical parameters either remained at the peak 3-day values or decreased toward normal levels. The biochemical changes are consistent with known reported CdCl2-induced edema and inflammation accompanied by phagocyte recruitment into lung tissue and reparative proliferation of lung cell types.
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U2 - 10.1016/0006-2952(79)90102-3
DO - 10.1016/0006-2952(79)90102-3
M3 - Article
VL - 28
SP - 381
EP - 388
JO - Biochemical Pharmacology
JF - Biochemical Pharmacology
SN - 0006-2952
IS - 3
ER -