Biochemical characterization of the DNA helicase activity of the Escherichia coli RecQ helicase

Frank G. Harmon, Stephen C. Kowalczykowski

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

We demonstrate that RecQ helicase from Escherichia coli is a catalytic helicase whose activity depends on the concentration of ATP, free magnesium ion, and singlestranded DNA-binding (SSB) protein. Helicase activity is cooperative in ATP concentration, with an apparent S0.5 value for ATP of 200 μM and a Hill coefficient of 3.3 ± 0.3. Therefore, RecQ helicase utilizes multiple, interacting ATP-binding sites to mediate double-stranded DNA (dsDNA) unwinding, implicating a multimer of at least three subunits as the active unwinding species. Unwinding activity is independent of dsDNA ends, indicating that RecQ helicase can unwind from both internal regions and ends of dsDNA. The KM for dsDNA is 0.5-0.9 μM base pairs; the kcat for DNA unwinding is 2.3-2.7 base pairs/s/monomer of RecQ helicase; and unexpectedly, helicase activity is optimal at a free magnesium ion concentration of 0.05 mM. Omitting Escherichia coli SSB protein lowers the rate and extent of dsDNA unwinding, suggesting that RecQ helicase associates with the single-stranded DNA (ssDNA) product. In agreement, the ssDNA-dependent ATPase activity is reduced in proportion to the SSB protein concentration; in its absence, ATPase activity saturates at six nucleotides/RecQ helicase monomer and yields a kcat of 24 s-1. Thus, we conclude that SSB protein stimulates RecQ helicase-mediated unwinding by both trapping the separated ssDNA strands after unwinding and preventing the formation of non-productive enzyme-ssDNA complexes.

Original languageEnglish (US)
Pages (from-to)232-243
Number of pages12
JournalJournal of Biological Chemistry
Volume276
Issue number1
DOIs
StatePublished - Jan 5 2001

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RecQ Helicases
Escherichia coli
DNA-Binding Proteins
Adenosine Triphosphate
Single-Stranded DNA
DNA
Base Pairing
Magnesium
Monomers
Ions
Adenosine Triphosphatases
Carrier Proteins
Nucleotides
Binding Sites

ASJC Scopus subject areas

  • Biochemistry

Cite this

Biochemical characterization of the DNA helicase activity of the Escherichia coli RecQ helicase. / Harmon, Frank G.; Kowalczykowski, Stephen C.

In: Journal of Biological Chemistry, Vol. 276, No. 1, 05.01.2001, p. 232-243.

Research output: Contribution to journalArticle

Harmon, Frank G. ; Kowalczykowski, Stephen C. / Biochemical characterization of the DNA helicase activity of the Escherichia coli RecQ helicase. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 1. pp. 232-243.
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