Biochemical characterization of Helicobacter pylori α1–3-fucosyltransferase and its application in the synthesis of fucosylated human milk oligosaccharides

Jing Bai, Zhigang Wu, Go Sugiarto, Madhusudhan Reddy Gadi, Hai Yu, Yanhong Li, Cong Xiao, Alice Ngo, Baohua Zhao, Xi Chen, Wanyi Guan

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Fucosylated human milk oligosaccharides (HMOs) have important biological functions. Enzymatic synthesis of such compounds requires robust fucosyltransferases. A C-terminal 66-amino acid truncated version of Helicobacter pylori α1–3-fucosyltransferase (Hp3FT) is a good candidate. Hp3FT was biochemically characterized to identify optimal conditions for enzymatic synthesis of fucosides. While N-acetyllactosamine (LacNAc) and lactose were both suitable acceptors, the former is preferred. At a low guanosine 5′-diphospho-β-L-fucose (GDP-Fuc) to acceptor ratio, Hp3FT selectively fucosylated LacNAc. Based on these enzymatic characteristics, diverse fucosylated HMOs, including 3-fucosyllactose (3-FL), lacto-N-fucopentaose (LNFP) III, lacto-N-neofucopentaose (LNnFP) V, lacto-N-neodifucohexaose (LNnDFH) II, difuco- and trifuco-para-lacto-N-neohexaose (DF-paraLNnH and TF-para-LNnH), were synthesized enzymatically by varying the ratio of the donor and acceptor as well as controlling the order of multiple glycosyltransferase-catalyzed reactions.

Original languageEnglish (US)
Pages (from-to)1-6
Number of pages6
JournalCarbohydrate Research
Volume480
DOIs
StatePublished - Jul 1 2019

Keywords

  • Enzymatic synthesis
  • Fucosyltransferase
  • Human milk oligosaccharide
  • Substrate specificity

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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