TY - JOUR
T1 - Biochemical characterization of a variant form of cytosolic epoxide hydrolase induced by parental exposure to N-ethyl-N-nitrosourea
AU - Nourooz-Zadeh, Jaffar
AU - Winder, Bruce S.
AU - Dietze, Eric C.
AU - Giometti, Carol S.
AU - Tollaksen, Sandra L.
AU - Hammock, Bruce D.
PY - 1992
Y1 - 1992
N2 - 1. ENU4 mice express a protein variant originally detected in a CBF1 mouse sired by a C57BL/6 mouse exposed to N-ethyl-N-nitrosourea. It appears to be an isolelectric point variant of cytosolic epoxide hydrolase. Affinity purified cytosolic epoxide hydrolase from ENU4 mice has a pI of approximately 5.1 compared to 5.6 in other mouse strains. 2. Clofibrate induced cytosolic epoxide hydrolase to similar levels in five strains of mice. However, CBF1 and ENU4 mice were more sensitive to the induction of palmitoyl CoA oxidase activity. 3. Except for isoelectric point, the physico- and immunochemical properties of cytosolic epoxide hydrolase from ENU4 mice were similar to those of the other mouse strains. Substrate specificities for five of six substrates tested were also similar.
AB - 1. ENU4 mice express a protein variant originally detected in a CBF1 mouse sired by a C57BL/6 mouse exposed to N-ethyl-N-nitrosourea. It appears to be an isolelectric point variant of cytosolic epoxide hydrolase. Affinity purified cytosolic epoxide hydrolase from ENU4 mice has a pI of approximately 5.1 compared to 5.6 in other mouse strains. 2. Clofibrate induced cytosolic epoxide hydrolase to similar levels in five strains of mice. However, CBF1 and ENU4 mice were more sensitive to the induction of palmitoyl CoA oxidase activity. 3. Except for isoelectric point, the physico- and immunochemical properties of cytosolic epoxide hydrolase from ENU4 mice were similar to those of the other mouse strains. Substrate specificities for five of six substrates tested were also similar.
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U2 - 10.1016/0742-8413(92)90253-4
DO - 10.1016/0742-8413(92)90253-4
M3 - Article
C2 - 1360372
AN - SCOPUS:0026787384
VL - 103
SP - 207
EP - 214
JO - Comparative biochemistry and physiology. C: Comparative pharmacology
JF - Comparative biochemistry and physiology. C: Comparative pharmacology
SN - 0742-8413
IS - 1
ER -