Biochemical changes caused by decellularization may compromise mechanical integrity of tracheal scaffolds

L. Partington, N. J. Mordan, C. Mason, J. C. Knowles, H. W. Kim, M. W. Lowdell, M. A. Birchall, I. B. Wall

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Tissue-engineered airways have achieved clinical success, but concerns remain about short-term loss of biomechanical properties, necessitating a stent. This study investigated the effect of chemical-enzymatic decellularization on biochemical properties of trachea important for cell attachment and vascularization (fibronectin and laminin) and cartilage matrix homeostasis (type II collagen and glycosaminoglycans (GAG)), as well as biomechanical status. Native trachea was used as a control, and NDC trachea stored in phosphate buffered saline (PBS) in parallel to decellularization was used as a time-matched control. Decellularization removed most cells, but chondrocytes and DNA remained after 25 cycles. Fibronectin was retained throughout the lamina propria and laminin at basement membranes. DNA accumulation along ECM fibres was seen. A decline in soluble collagen was observed in decellularized tissue. GAG content of cartilage rings was reduced, even in PBS control tissue from 20 cycles onwards (p < 0.05), but decellularization caused the greatest loss (p < 0.01). Tensile strength declined throughout the process, but was significant only at later time points. The data demonstrate that the substantial reduction in GAG might contribute to loss of mechanical integrity of biotracheas. Overcoming structural changes that cause an imbalance in cartilage matrix equilibrium will be necessary to optimize clinical benefit, enabling widespread use of biotracheas.

Original languageEnglish (US)
Pages (from-to)5251-5261
Number of pages11
JournalActa Biomaterialia
Volume9
Issue number2
DOIs
StatePublished - Feb 2013

Fingerprint

Cartilage
Trachea
Glycosaminoglycans
Scaffolds
Laminin
Tissue
Fibronectins
Collagen
Phosphates
DNA
Military electronic countermeasures
Stents
Collagen Type II
Tensile Strength
Chondrocytes
Basement Membrane
Mucous Membrane
Homeostasis
Tensile strength
Fibers

Keywords

  • Glycosaminoglycans
  • Mechanical properties
  • Scaffold
  • Tracheal prosthesis

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Biotechnology
  • Biochemistry
  • Molecular Biology

Cite this

Partington, L., Mordan, N. J., Mason, C., Knowles, J. C., Kim, H. W., Lowdell, M. W., ... Wall, I. B. (2013). Biochemical changes caused by decellularization may compromise mechanical integrity of tracheal scaffolds. Acta Biomaterialia, 9(2), 5251-5261. https://doi.org/10.1016/j.actbio.2012.10.004

Biochemical changes caused by decellularization may compromise mechanical integrity of tracheal scaffolds. / Partington, L.; Mordan, N. J.; Mason, C.; Knowles, J. C.; Kim, H. W.; Lowdell, M. W.; Birchall, M. A.; Wall, I. B.

In: Acta Biomaterialia, Vol. 9, No. 2, 02.2013, p. 5251-5261.

Research output: Contribution to journalArticle

Partington, L, Mordan, NJ, Mason, C, Knowles, JC, Kim, HW, Lowdell, MW, Birchall, MA & Wall, IB 2013, 'Biochemical changes caused by decellularization may compromise mechanical integrity of tracheal scaffolds', Acta Biomaterialia, vol. 9, no. 2, pp. 5251-5261. https://doi.org/10.1016/j.actbio.2012.10.004
Partington, L. ; Mordan, N. J. ; Mason, C. ; Knowles, J. C. ; Kim, H. W. ; Lowdell, M. W. ; Birchall, M. A. ; Wall, I. B. / Biochemical changes caused by decellularization may compromise mechanical integrity of tracheal scaffolds. In: Acta Biomaterialia. 2013 ; Vol. 9, No. 2. pp. 5251-5261.
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