Biochemical and immunological comparisons of carbohydrate-binding protein 35 and an IgE-binding protein.

J. G. Laing, M. W. Robertson, C. A. Gritzmacher, J. L. Wang, Fu-Tong Liu

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47 Scopus citations

Abstract

The predicted amino acid sequence of carbohydrate-binding protein 35 (CBP35; Mr approximately 35,000), a galactose-specific lectin in many mouse and human cells, has been compared to the predicted sequence of an IgE-binding protein (epsilon BP) originally identified in rat basophilic leukemia cells. The sequences of the two proteins showed that: (a) 85% of the amino acid residues are identical; (b) the polypeptide chains are comprised of two distinct domains; and (c) highly conserved internal repetitive sequences are present. Consistent with these observations, antisera raised against CBP35 or against a synthetic peptide derived from the epsilon BP sequence cross-reacted with both proteins. Moreover, fractionation of extracts of mouse 3T3 fibroblasts over an IgE-Sepharose affinity column showed that CBP35 bound to IgE; this binding was reversed by addition of lactose. Conversely, fractionation of extracts of rat basophilic leukemia cells over an affinity column of Sepharose derivatized with N-(epsilon-amino-caproyl)-D-galactosamine showed that epsilon BP was a galactose-binding protein. Furthermore, epsilon BP bound to IgE-Sepharose could be eluted by lactose. Finally, transcription and translation in vitro of the cDNA corresponding to epsilon BP yielded a polypeptide containing carbohydrate-binding activity. All of these results strongly support the conclusion that CBP35 and epsilon BP are mouse and rat homologues, respectively.

Original languageEnglish (US)
Pages (from-to)1097-1010
Number of pages88
JournalJournal of Biological Chemistry
Volume264
Issue number4
StatePublished - Feb 5 1989
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

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  • Cite this

    Laing, J. G., Robertson, M. W., Gritzmacher, C. A., Wang, J. L., & Liu, F-T. (1989). Biochemical and immunological comparisons of carbohydrate-binding protein 35 and an IgE-binding protein. Journal of Biological Chemistry, 264(4), 1097-1010.