Biochemical and immunological characterization of the major structural proteins of feline immunodeficiency virus

R. Steinman, J. Dombrowski, T. O'Connor, R. C. Montelaro, Q. Tonelli, K. Lawrence, C. Seymour, J. Goodness, Niels C Pedersen, P. R. Andersen

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Feline immunodeficiency virus (FIV) structural proteins were identified using sera obtained from experimentally inoculated cats. Proteins analysed by both radioimmunoprecipitation and Western blotting were specific for FIV infection and failed to cross-react with either antisera to feline leukaemia virus of feline syncytium-forming virus. Western blot analysis of purified virus revealed immunoreactive proteins with apparent M(r) of 65K, 50K, 40K, 32K, 24K, 15K and 10K. The major core structural proteins of the virus were isolated by reverse phase HPLC and the amino-terminal sequences of p10 and p24 were determined. Monoclonal antibodies specific for p24 suggested the presence of a precursor protein that could be detected in 35[S]methionine/cysteine-labelled, virus-infected cell extracts. This putative precursor protein possessed an apparent M(r) of 50K (Pr50(gag)). Further analysis revealed the presence of two additional proteins of 130K and 40K. Experiments utilizing tunicamycin, endoglycosidase H and glycopeptidase F revealed that p130 and p40 exhibited properties characteristic of glycoproteins. Our studies also indicated that FIV is immunologically related to other lentiviruses.

Original languageEnglish (US)
Pages (from-to)701-706
Number of pages6
JournalJournal of General Virology
Volume71
Issue number3
StatePublished - 1990
Externally publishedYes

Fingerprint

Feline Immunodeficiency Virus
Protein Precursors
Viruses
Spumavirus
Western Blotting
Feline Leukemia Virus
Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase
Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase
Viral Core Proteins
Viral Structural Proteins
Tunicamycin
Lentivirus
Proteins
Virus Diseases
Cell Extracts
Methionine
Cysteine
Immune Sera
Glycoproteins
Cats

ASJC Scopus subject areas

  • Virology
  • Immunology

Cite this

Steinman, R., Dombrowski, J., O'Connor, T., Montelaro, R. C., Tonelli, Q., Lawrence, K., ... Andersen, P. R. (1990). Biochemical and immunological characterization of the major structural proteins of feline immunodeficiency virus. Journal of General Virology, 71(3), 701-706.

Biochemical and immunological characterization of the major structural proteins of feline immunodeficiency virus. / Steinman, R.; Dombrowski, J.; O'Connor, T.; Montelaro, R. C.; Tonelli, Q.; Lawrence, K.; Seymour, C.; Goodness, J.; Pedersen, Niels C; Andersen, P. R.

In: Journal of General Virology, Vol. 71, No. 3, 1990, p. 701-706.

Research output: Contribution to journalArticle

Steinman, R, Dombrowski, J, O'Connor, T, Montelaro, RC, Tonelli, Q, Lawrence, K, Seymour, C, Goodness, J, Pedersen, NC & Andersen, PR 1990, 'Biochemical and immunological characterization of the major structural proteins of feline immunodeficiency virus', Journal of General Virology, vol. 71, no. 3, pp. 701-706.
Steinman R, Dombrowski J, O'Connor T, Montelaro RC, Tonelli Q, Lawrence K et al. Biochemical and immunological characterization of the major structural proteins of feline immunodeficiency virus. Journal of General Virology. 1990;71(3):701-706.
Steinman, R. ; Dombrowski, J. ; O'Connor, T. ; Montelaro, R. C. ; Tonelli, Q. ; Lawrence, K. ; Seymour, C. ; Goodness, J. ; Pedersen, Niels C ; Andersen, P. R. / Biochemical and immunological characterization of the major structural proteins of feline immunodeficiency virus. In: Journal of General Virology. 1990 ; Vol. 71, No. 3. pp. 701-706.
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