Biochemical and histochemical properties of hepatic tumors of rainbow trout, Oncorhynchus mykiss

Lynn M. Parker, Darrel J. Laurén, Bruce D. Hammock, Bruce Winder, David E. Hinton

Research output: Contribution to journalArticle

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Abstract

Biochemical and histochemical studies were conducted in aflatoxin B1-induced liver tumors in adult rainbow trout. Specific activities of the phase I enzymes, ethoxyresorufin-O-deethylase (EROD), microsomal and cytosolic epoxide hydrolase (mEH and cEH), aldehyde dehydrogenase (ALDH) and DT-diaphorase, and the phase II enzymes, γ-glutamyltransferase (γ-GT), glutathione transferase (GST) and uridine diphosphoglucuronyl transferase (UDPGT) were measured. Cryostat sections of tumor and surrounding liver from the same cohorts were analyzed immunohistochemically for cytochrome P450IA1 and histochemically for ALDH (benzaldehyde and hexanal), DT-diaphorase, γ-GT and uridine diphosphoglucuronyl dehydrogenase (UDPGdH). In tumor tissues, the largest biochemical changes were found with benzaldehyde dehydrogenase, where activity increased from undetectable levels to 7.4 nmol/min/mg protein, and γ-GT, where activity increased 12-fold over controls. Increases in other enzymes ranged from 1.26 to 2.84 times that of control liver, except EROD, which decreased, and cEH and mEH, which were unchanged. Histochemical analyses showed the induction of ALDH, γ-GT, DT-diaphorase and UDPGdH, and the depression of cytochrome P450IA1 in hepatic neoplasms. In addition, marker enzyme histochemistry of neoplasms revealed heterogeneous populations of hepatocytes and absence of necrotic areas.

Original languageEnglish (US)
Pages (from-to)211-217
Number of pages7
JournalCarcinogenesis
Volume14
Issue number2
StatePublished - Feb 1993

Fingerprint

Oncorhynchus mykiss
NAD(P)H Dehydrogenase (Quinone)
Aldehyde Dehydrogenase
Tumors
Uridine
Tumor
Enzymes
Liver
Oxidoreductases
Cytochrome P-450 CYP1A1
Cytochromes
Aldehydes
Neoplasms
Histochemistry
Proteins
Epoxide Hydrolases
Aflatoxin B1
Glutathione
Liver Neoplasms
Transferases

ASJC Scopus subject areas

  • Cancer Research
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Physiology
  • Behavioral Neuroscience

Cite this

Parker, L. M., Laurén, D. J., Hammock, B. D., Winder, B., & Hinton, D. E. (1993). Biochemical and histochemical properties of hepatic tumors of rainbow trout, Oncorhynchus mykiss. Carcinogenesis, 14(2), 211-217.

Biochemical and histochemical properties of hepatic tumors of rainbow trout, Oncorhynchus mykiss. / Parker, Lynn M.; Laurén, Darrel J.; Hammock, Bruce D.; Winder, Bruce; Hinton, David E.

In: Carcinogenesis, Vol. 14, No. 2, 02.1993, p. 211-217.

Research output: Contribution to journalArticle

Parker, LM, Laurén, DJ, Hammock, BD, Winder, B & Hinton, DE 1993, 'Biochemical and histochemical properties of hepatic tumors of rainbow trout, Oncorhynchus mykiss', Carcinogenesis, vol. 14, no. 2, pp. 211-217.
Parker LM, Laurén DJ, Hammock BD, Winder B, Hinton DE. Biochemical and histochemical properties of hepatic tumors of rainbow trout, Oncorhynchus mykiss. Carcinogenesis. 1993 Feb;14(2):211-217.
Parker, Lynn M. ; Laurén, Darrel J. ; Hammock, Bruce D. ; Winder, Bruce ; Hinton, David E. / Biochemical and histochemical properties of hepatic tumors of rainbow trout, Oncorhynchus mykiss. In: Carcinogenesis. 1993 ; Vol. 14, No. 2. pp. 211-217.
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