Biochemical and biophysical characterization of human recombinant IgE-binding protein, an S-type animal lectin

Daniel K. Hsu, Riaz I. Zuberi, Fu-Tong Liu

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Abstract

IgE-binding protein (εBP) was originally identified by virtue of its affinity for IgE. It is now known to be a β-galactoside-binding lectin with the characteristic of an S-type carbohydrate recognition domain. The protein is composed of two domains: the amino-terminal domain consisting of tandem repeats and the carboxyl-terminal domain containing sequences shared by other S-type carbohydrate recognition domains. The amino-terminal domain also contains a number of potential recognition sites for collagenase cleavage. In this study, human εBP was first expressed in Escherichia coli, and the carboxyl-terminal domain (εBP-C) was then generated by collagenase digestion of εBP. By equilibrium dialysis, the association constants of εBP and εBP-C for lactose were found to be similar (6.0 ± 0.70) x 104 M-1 and (4.7 ± 0.27) x 104 M-1, respectively. Both polypeptides contain only one lactose-binding site/molecule. By an assay involving binding of 125I-labeled eBP or εBP-C to solid phase IgE, and inhibition of this binding by saccharides, it was determined that εBP-C retains the saccharide specificity of εBP. Importantly, although unlabeled εBP-C inhibited the binding of the radiolabeled εBP to IgE, unlabeled εBP caused increased binding to IgE, suggesting self-association among εBP molecules. Oligomeric structures resulting from self-association of εBP were confirmed by chemical cross-linking studies. Furthermore, εBP possesses hemagglutination activity on rabbit erythrocytes, whereas εBP-C lacks such activity. Based on these results, we propose a structural model for multivalency of εBP: dimerization or oligomerization of εBP occurs through intermolecular interaction involving the amino-terminal domain.

Original languageEnglish (US)
Pages (from-to)14167-14174
Number of pages8
JournalJournal of Biological Chemistry
Volume267
Issue number20
StatePublished - Jul 15 1992

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Galectins
Galectin 3
Protein S
Lectins
Carrier Proteins
Protein C
Immunoglobulin E
Collagenases
Lactose
Association reactions
Protein Multimerization
Carbohydrates
Galactosides
Tandem Repeat Sequences
Structural Models
Hemagglutination
Oligomerization
Molecules
Protein Binding
Dialysis

ASJC Scopus subject areas

  • Biochemistry

Cite this

Biochemical and biophysical characterization of human recombinant IgE-binding protein, an S-type animal lectin. / Hsu, Daniel K.; Zuberi, Riaz I.; Liu, Fu-Tong.

In: Journal of Biological Chemistry, Vol. 267, No. 20, 15.07.1992, p. 14167-14174.

Research output: Contribution to journalArticle

Hsu, Daniel K. ; Zuberi, Riaz I. ; Liu, Fu-Tong. / Biochemical and biophysical characterization of human recombinant IgE-binding protein, an S-type animal lectin. In: Journal of Biological Chemistry. 1992 ; Vol. 267, No. 20. pp. 14167-14174.
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