Biochemical and antigenic properties of the first isolates of infectious hematopoietic necrosis virus from salmonid fish in europe

K. D. Arkush, G. Bovo, P. De Klnkelin, J. R. Wlnton, W. H. Wlngfield, Ronald Hedrick

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Abstract

The first isolates of infectious hematopoietic necrosis virus (IHNV) recovered from rainbow trout Oncorhynchus mykiss (formerly Salmo gairdneri) in France and Italy were compared to six representative strains from North America by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of virion polypeptides and neutralization by monoclonal antibodies (MAbs). All three IHNV isolates from Europe had similar polypeptide profiles when compared by SDS-PAGE. An analysis of the antigenic relatedness of the European isolates to representative strains from North America showed that they were clearly different from viruses obtained from salmonids in California. The RB/B5 MAb, which was developed against virus isolated from adult steelhead (anadromous rainbow trout) reared in central Oregon, neutralized all isolates examined. The 193-110/B4 MAb, developed against IHNV isolated from infected yearling rainbow trout in southern Idaho, neutralized all isolates tested except those from California. The SRCV/A4 MAb, developed against Sacramento River chinook virus (SRCV) isolated from adult spring chinook salmon O. tshawytscha in central California, was the least reactive, and strong neutralization was observed only with the SRCV strain of IHNV from California. However, partial reactivity of the vims isolates from France with the SRCV/A4 MAb distinguished them from the virus recovered from salmonids in Italy.

Original languageEnglish (US)
Pages (from-to)148-153
Number of pages6
JournalJournal of Aquatic Animal Health
Volume1
Issue number2
DOIs
StatePublished - Jan 1 1989

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Infectious hematopoietic necrosis virus
salmonid
virus
Sacramento River
viruses
Oncorhynchus mykiss
fish
Salmonidae
neutralization
polyacrylamide gel electrophoresis
rainbow
polypeptides
France
Italy
river
Oncorhynchus tshawytscha
electrokinesis
Europe
necrosis
gel

ASJC Scopus subject areas

  • Aquatic Science

Cite this

Biochemical and antigenic properties of the first isolates of infectious hematopoietic necrosis virus from salmonid fish in europe. / Arkush, K. D.; Bovo, G.; Klnkelin, P. De; Wlnton, J. R.; Wlngfield, W. H.; Hedrick, Ronald.

In: Journal of Aquatic Animal Health, Vol. 1, No. 2, 01.01.1989, p. 148-153.

Research output: Contribution to journalArticle

Arkush, K. D. ; Bovo, G. ; Klnkelin, P. De ; Wlnton, J. R. ; Wlngfield, W. H. ; Hedrick, Ronald. / Biochemical and antigenic properties of the first isolates of infectious hematopoietic necrosis virus from salmonid fish in europe. In: Journal of Aquatic Animal Health. 1989 ; Vol. 1, No. 2. pp. 148-153.
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