Abstract
The first isolates of infectious hematopoietic necrosis virus (IHNV) recovered from rainbow trout Oncorhynchus mykiss (formerly Salmo gairdneri) in France and Italy were compared to six representative strains from North America by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of virion polypeptides and neutralization by monoclonal antibodies (MAbs). All three IHNV isolates from Europe had similar polypeptide profiles when compared by SDS-PAGE. An analysis of the antigenic relatedness of the European isolates to representative strains from North America showed that they were clearly different from viruses obtained from salmonids in California. The RB/B5 MAb, which was developed against virus isolated from adult steelhead (anadromous rainbow trout) reared in central Oregon, neutralized all isolates examined. The 193-110/B4 MAb, developed against IHNV isolated from infected yearling rainbow trout in southern Idaho, neutralized all isolates tested except those from California. The SRCV/A4 MAb, developed against Sacramento River chinook virus (SRCV) isolated from adult spring chinook salmon O. tshawytscha in central California, was the least reactive, and strong neutralization was observed only with the SRCV strain of IHNV from California. However, partial reactivity of the vims isolates from France with the SRCV/A4 MAb distinguished them from the virus recovered from salmonids in Italy.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 148-153 |
| Number of pages | 6 |
| Journal | Journal of Aquatic Animal Health |
| Volume | 1 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jan 1 1989 |
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ASJC Scopus subject areas
- Aquatic Science
Cite this
Biochemical and antigenic properties of the first isolates of infectious hematopoietic necrosis virus from salmonid fish in europe. / Arkush, K. D.; Bovo, G.; Klnkelin, P. De; Wlnton, J. R.; Wlngfield, W. H.; Hedrick, Ronald.
In: Journal of Aquatic Animal Health, Vol. 1, No. 2, 01.01.1989, p. 148-153.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Biochemical and antigenic properties of the first isolates of infectious hematopoietic necrosis virus from salmonid fish in europe
AU - Arkush, K. D.
AU - Bovo, G.
AU - Klnkelin, P. De
AU - Wlnton, J. R.
AU - Wlngfield, W. H.
AU - Hedrick, Ronald
PY - 1989/1/1
Y1 - 1989/1/1
N2 - The first isolates of infectious hematopoietic necrosis virus (IHNV) recovered from rainbow trout Oncorhynchus mykiss (formerly Salmo gairdneri) in France and Italy were compared to six representative strains from North America by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of virion polypeptides and neutralization by monoclonal antibodies (MAbs). All three IHNV isolates from Europe had similar polypeptide profiles when compared by SDS-PAGE. An analysis of the antigenic relatedness of the European isolates to representative strains from North America showed that they were clearly different from viruses obtained from salmonids in California. The RB/B5 MAb, which was developed against virus isolated from adult steelhead (anadromous rainbow trout) reared in central Oregon, neutralized all isolates examined. The 193-110/B4 MAb, developed against IHNV isolated from infected yearling rainbow trout in southern Idaho, neutralized all isolates tested except those from California. The SRCV/A4 MAb, developed against Sacramento River chinook virus (SRCV) isolated from adult spring chinook salmon O. tshawytscha in central California, was the least reactive, and strong neutralization was observed only with the SRCV strain of IHNV from California. However, partial reactivity of the vims isolates from France with the SRCV/A4 MAb distinguished them from the virus recovered from salmonids in Italy.
AB - The first isolates of infectious hematopoietic necrosis virus (IHNV) recovered from rainbow trout Oncorhynchus mykiss (formerly Salmo gairdneri) in France and Italy were compared to six representative strains from North America by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of virion polypeptides and neutralization by monoclonal antibodies (MAbs). All three IHNV isolates from Europe had similar polypeptide profiles when compared by SDS-PAGE. An analysis of the antigenic relatedness of the European isolates to representative strains from North America showed that they were clearly different from viruses obtained from salmonids in California. The RB/B5 MAb, which was developed against virus isolated from adult steelhead (anadromous rainbow trout) reared in central Oregon, neutralized all isolates examined. The 193-110/B4 MAb, developed against IHNV isolated from infected yearling rainbow trout in southern Idaho, neutralized all isolates tested except those from California. The SRCV/A4 MAb, developed against Sacramento River chinook virus (SRCV) isolated from adult spring chinook salmon O. tshawytscha in central California, was the least reactive, and strong neutralization was observed only with the SRCV strain of IHNV from California. However, partial reactivity of the vims isolates from France with the SRCV/A4 MAb distinguished them from the virus recovered from salmonids in Italy.
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U2 - 10.1080/1548-8667(1989)001[lt]0148[co]BAAPOT[gt]2.3.CO;2
DO - 10.1080/1548-8667(1989)001[lt]0148[co]BAAPOT[gt]2.3.CO;2
M3 - Article
VL - 1
SP - 148
EP - 153
JO - Journal of Aquatic Animal Health
JF - Journal of Aquatic Animal Health
SN - 0899-7659
IS - 2
ER -