Biocatalytic conversion of ethylene to ethylene oxide using an engineered toluene monooxygenase

D. A. Carlin, S. J. Bertolani, Justin Siegel

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Mutants of toluene o-xylene monooxygenase are demonstrated to oxidize ethylene to ethylene oxide in vivo at yields of >99%. The best mutant increases ethylene oxidation activity by >5500-fold relative to the native enzyme. This is the first report of a recombinant enzyme capable of carrying out this industrially significant chemical conversion.

Original languageEnglish (US)
Pages (from-to)2283-2285
Number of pages3
JournalChemical Communications
Volume51
Issue number12
DOIs
StatePublished - Feb 11 2015

Fingerprint

Ethylene Oxide
Toluene
Mixed Function Oxygenases
Ethylene
Oxides
Enzymes
Xylene
Oxidation
ethylene
toluene 2-xylene monooxygenase

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Ceramics and Composites
  • Electronic, Optical and Magnetic Materials
  • Surfaces, Coatings and Films
  • Materials Chemistry
  • Metals and Alloys

Cite this

Biocatalytic conversion of ethylene to ethylene oxide using an engineered toluene monooxygenase. / Carlin, D. A.; Bertolani, S. J.; Siegel, Justin.

In: Chemical Communications, Vol. 51, No. 12, 11.02.2015, p. 2283-2285.

Research output: Contribution to journalArticle

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