BIOASSAY OF ANTI JUVENILE HORMONE COMPOUNDS: AN ALTERNATIVE APPROACH.

Thomas C. Sparks, R. Michael Roe, Adrian Buehler, Bruce D. Hammock

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

During the last larval stadium of the cabbage looper, Trichoplusia ni, Hubner, the prepupal burst of juvenile hormone (JH) stimulates the appearance of juvenile hormone esterase (JHE) which, in turn, degrades the JH. Disruption of this prepupal burst of JH by anti-juvenile hormones (AJHs) such as fluoromevalonolactone, result in a variety of teratogenic effects including delayed tanning and/or pupation, malformed larvae and reduced JHE activity. Although general toxicants and esterase inhibitors may also cause malformed larvae, there is usually no delay in tanning and/or pupation. These observations provide the basis for a simple, rapid AJH bioassay using an economically important pest insect (T. ni). A simple 'key' was devised to help facilitate the use of this bioassay.

Original languageEnglish (US)
Title of host publicationACS Symposium Series
Place of PublicationWashington, DC, USA
PublisherACS
Pages293-306
Number of pages14
ISBN (Print)0841209103
StatePublished - 1985
Externally publishedYes

Fingerprint

Juvenile Hormones
Bioassay
Tanning
Stadiums
Esterases

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Sparks, T. C., Roe, R. M., Buehler, A., & Hammock, B. D. (1985). BIOASSAY OF ANTI JUVENILE HORMONE COMPOUNDS: AN ALTERNATIVE APPROACH. In ACS Symposium Series (pp. 293-306). Washington, DC, USA: ACS.

BIOASSAY OF ANTI JUVENILE HORMONE COMPOUNDS : AN ALTERNATIVE APPROACH. / Sparks, Thomas C.; Roe, R. Michael; Buehler, Adrian; Hammock, Bruce D.

ACS Symposium Series. Washington, DC, USA : ACS, 1985. p. 293-306.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Sparks, TC, Roe, RM, Buehler, A & Hammock, BD 1985, BIOASSAY OF ANTI JUVENILE HORMONE COMPOUNDS: AN ALTERNATIVE APPROACH. in ACS Symposium Series. ACS, Washington, DC, USA, pp. 293-306.
Sparks TC, Roe RM, Buehler A, Hammock BD. BIOASSAY OF ANTI JUVENILE HORMONE COMPOUNDS: AN ALTERNATIVE APPROACH. In ACS Symposium Series. Washington, DC, USA: ACS. 1985. p. 293-306
Sparks, Thomas C. ; Roe, R. Michael ; Buehler, Adrian ; Hammock, Bruce D. / BIOASSAY OF ANTI JUVENILE HORMONE COMPOUNDS : AN ALTERNATIVE APPROACH. ACS Symposium Series. Washington, DC, USA : ACS, 1985. pp. 293-306
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