BIN1 Induces the Formation of T-Tubules and Adult-Like Ca2+ Release Units in Developing Cardiomyocytes

Ana De La Mata, Sendoa Tajada, Samantha O'Dwyer, Collin Matsumoto, Rose Ellen Dickson, Nirmala Hariharan, Claudia M. Moreno, Luis Fernando Santana

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Human embryonic stem cell-derived cardiomyocytes (hESC-CMs) are at the center of new cell-based therapies for cardiac disease, but may also serve as a useful in vitro model for cardiac cell development. An intriguing feature of hESC-CMs is that although they express contractile proteins and have sarcomeres, they do not develop transverse-tubules (T-tubules) with adult-like Ca2+ release units (CRUs). We tested the hypothesis that expression of the protein BIN1 in hESC-CMs promotes T-tubules formation, facilitates CaV1.2 channel clustering along the tubules, and results in the development of stable CRUs. Using electrophysiology, [Ca2+]i imaging, and super resolution microscopy, we found that BIN1 expression induced T-tubule development in hESC-CMs, while increasing differentiation toward a more ventricular-like phenotype. Voltage-gated CaV1.2 channels clustered along the surface sarcolemma and T-tubules of hESC-CM. The length and width of the T-tubules as well as the expression and size of CaV1.2 clusters grew, as BIN1 expression increased and cells matured. BIN1 expression increased CaV1.2 channel activity and the probability of coupled gating within channel clusters. Interestingly, BIN1 clusters also served as sites for sarcoplasmic reticulum (SR) anchoring and stabilization. Accordingly, BIN1-expressing cells had more CaV1.2-ryanodine receptor junctions than control cells. This was associated with larger [Ca2+]i transients during excitation–contraction coupling. Our data support the view that BIN1 is a key regulator of T-tubule formation and CaV1.2 channel delivery. By studying the role of BIN1 during the differentiation of hESC-CMs, we show that BIN1 is also important for CaV1.2 channel clustering, junctional SR organization, and the establishment of excitation–contraction coupling. Stem Cells 2018.

Original languageEnglish (US)
JournalStem Cells
DOIs
StateAccepted/In press - Jan 1 2018

Fingerprint

Cardiac Myocytes
Sarcoplasmic Reticulum
Cluster Analysis
Contractile Proteins
Sarcolemma
Sarcomeres
Ryanodine Receptor Calcium Release Channel
Intercellular Junctions
Electrophysiology
Cell- and Tissue-Based Therapy
Human Embryonic Stem Cells
Microscopy
Heart Diseases
Stem Cells
Phenotype
Proteins

Keywords

  • BIN1
  • Ca1.2
  • Calcium release units
  • Cardiac myocytes
  • hESC
  • T-tubules

ASJC Scopus subject areas

  • Molecular Medicine
  • Developmental Biology
  • Cell Biology

Cite this

BIN1 Induces the Formation of T-Tubules and Adult-Like Ca2+ Release Units in Developing Cardiomyocytes. / De La Mata, Ana; Tajada, Sendoa; O'Dwyer, Samantha; Matsumoto, Collin; Dickson, Rose Ellen; Hariharan, Nirmala; Moreno, Claudia M.; Santana, Luis Fernando.

In: Stem Cells, 01.01.2018.

Research output: Contribution to journalArticle

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abstract = "Human embryonic stem cell-derived cardiomyocytes (hESC-CMs) are at the center of new cell-based therapies for cardiac disease, but may also serve as a useful in vitro model for cardiac cell development. An intriguing feature of hESC-CMs is that although they express contractile proteins and have sarcomeres, they do not develop transverse-tubules (T-tubules) with adult-like Ca2+ release units (CRUs). We tested the hypothesis that expression of the protein BIN1 in hESC-CMs promotes T-tubules formation, facilitates CaV1.2 channel clustering along the tubules, and results in the development of stable CRUs. Using electrophysiology, [Ca2+]i imaging, and super resolution microscopy, we found that BIN1 expression induced T-tubule development in hESC-CMs, while increasing differentiation toward a more ventricular-like phenotype. Voltage-gated CaV1.2 channels clustered along the surface sarcolemma and T-tubules of hESC-CM. The length and width of the T-tubules as well as the expression and size of CaV1.2 clusters grew, as BIN1 expression increased and cells matured. BIN1 expression increased CaV1.2 channel activity and the probability of coupled gating within channel clusters. Interestingly, BIN1 clusters also served as sites for sarcoplasmic reticulum (SR) anchoring and stabilization. Accordingly, BIN1-expressing cells had more CaV1.2-ryanodine receptor junctions than control cells. This was associated with larger [Ca2+]i transients during excitation–contraction coupling. Our data support the view that BIN1 is a key regulator of T-tubule formation and CaV1.2 channel delivery. By studying the role of BIN1 during the differentiation of hESC-CMs, we show that BIN1 is also important for CaV1.2 channel clustering, junctional SR organization, and the establishment of excitation–contraction coupling. Stem Cells 2018.",
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AU - Tajada, Sendoa

AU - O'Dwyer, Samantha

AU - Matsumoto, Collin

AU - Dickson, Rose Ellen

AU - Hariharan, Nirmala

AU - Moreno, Claudia M.

AU - Santana, Luis Fernando

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AB - Human embryonic stem cell-derived cardiomyocytes (hESC-CMs) are at the center of new cell-based therapies for cardiac disease, but may also serve as a useful in vitro model for cardiac cell development. An intriguing feature of hESC-CMs is that although they express contractile proteins and have sarcomeres, they do not develop transverse-tubules (T-tubules) with adult-like Ca2+ release units (CRUs). We tested the hypothesis that expression of the protein BIN1 in hESC-CMs promotes T-tubules formation, facilitates CaV1.2 channel clustering along the tubules, and results in the development of stable CRUs. Using electrophysiology, [Ca2+]i imaging, and super resolution microscopy, we found that BIN1 expression induced T-tubule development in hESC-CMs, while increasing differentiation toward a more ventricular-like phenotype. Voltage-gated CaV1.2 channels clustered along the surface sarcolemma and T-tubules of hESC-CM. The length and width of the T-tubules as well as the expression and size of CaV1.2 clusters grew, as BIN1 expression increased and cells matured. BIN1 expression increased CaV1.2 channel activity and the probability of coupled gating within channel clusters. Interestingly, BIN1 clusters also served as sites for sarcoplasmic reticulum (SR) anchoring and stabilization. Accordingly, BIN1-expressing cells had more CaV1.2-ryanodine receptor junctions than control cells. This was associated with larger [Ca2+]i transients during excitation–contraction coupling. Our data support the view that BIN1 is a key regulator of T-tubule formation and CaV1.2 channel delivery. By studying the role of BIN1 during the differentiation of hESC-CMs, we show that BIN1 is also important for CaV1.2 channel clustering, junctional SR organization, and the establishment of excitation–contraction coupling. Stem Cells 2018.

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