Biliverdin reduction by cyanobacterial phycocyanobilin: ferredoxin oxidoreductase (PcyA) proceeds via linear tetrapyrrole radical intermediates

Shih Long Tu, Alexander Gunn, Michael D. Toney, R. David Britt, J. Clark Lagarias

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Cyanobacterial phycocyanobilin:ferredoxin oxidoreductase (PcyA) catalyzes the four electron reduction of biliverdin IXα (BV) to phycocyanobilin, a key step in the biosynthesis of the linear tetrapyrrole (bilin) prosthetic groups of cyanobacterial phytochromes and the light-harvesting phycobiliproteins. Using an anaerobic assay protocol, optically detected bilin-protein intermediates, produced during the PcyA catalytic cycle, were shown to correlate well with the appearance and decay of an isotropic g ≈ 2 EPR signal measured at low temperature. Absorption spectral simulations of biliverdin XIIIα reduction support a mechanism involving direct electron transfers from ferredoxin to protonated bilin:PcyA complexes.

Original languageEnglish (US)
Pages (from-to)8682-8693
Number of pages12
JournalJournal of the American Chemical Society
Volume126
Issue number28
DOIs
StatePublished - Jul 21 2004

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Tetrapyrroles
Biliverdine
Ferredoxins
Biosynthesis
Oxidoreductases
Phycobiliproteins
Electrons
Phytochrome
Prosthetics
Paramagnetic resonance
Assays
Proteins
Light
Temperature
phycocyanobilin

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Biliverdin reduction by cyanobacterial phycocyanobilin : ferredoxin oxidoreductase (PcyA) proceeds via linear tetrapyrrole radical intermediates. / Tu, Shih Long; Gunn, Alexander; Toney, Michael D.; Britt, R. David; Lagarias, J. Clark.

In: Journal of the American Chemical Society, Vol. 126, No. 28, 21.07.2004, p. 8682-8693.

Research output: Contribution to journalArticle

Tu, Shih Long ; Gunn, Alexander ; Toney, Michael D. ; Britt, R. David ; Lagarias, J. Clark. / Biliverdin reduction by cyanobacterial phycocyanobilin : ferredoxin oxidoreductase (PcyA) proceeds via linear tetrapyrrole radical intermediates. In: Journal of the American Chemical Society. 2004 ; Vol. 126, No. 28. pp. 8682-8693.
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