Bile-acid-mediated decrease in endoplasmic reticulum stress

A potential contributor to the metabolic benefits of ileal interposition surgery in UCD-T2DM rats

Bethany P. Cummings, Ahmed Bettaieb, James L. Graham, Jaehyoung Kim, Fangrui Ma, Noreene Shibata, Kimber Stanhope, Cecilia R Giulivi, Frederik Hansen, Jacob Jelsing, Niels Vrang, Mark Kowala, Michael L. Chouinard, Fawaz Haj, Peter J Havel

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Post-operative increases in circulating bile acids have been suggested to contribute to the metabolic benefits of bariatric surgery; however, their mechanistic contributions remain undefined. We have previously reported that ileal interposition (IT) surgery delays the onset of type 2 diabetes in UCD-T2DM rats and increases circulating bile acids, independently of effects on energy intake or body weight. Therefore, we investigated potential mechanisms by which post-operative increases in circulating bile acids improve glucose homeostasis after IT surgery. IT, sham or no surgery was performed on 2-month-old weight-matched male UCD-T2DM rats. Animals underwent an oral fat tolerance test (OFTT) and serial oral glucose tolerance tests (OGTT). Tissues were collected at 1.5 and 4.5 months after surgery. Cell culture models were used to investigate interactions between bile acids and ER stress. IT-operated animals exhibited marked improvements in glucose and lipid metabolism, with concurrent increases in postprandial glucagon-like peptide-1 (GLP-1) secretion during the OFTT and OGTTs, independently of food intake and body weight. Measurement of circulating bile acid profiles revealed increases in circulating total bile acids in IT-operated animals, with a preferential increase in circulating cholic acid concentrations. Gut microbial populations were assessed as potential contributors to the increases in circulating bile acid concentrations, which revealed proportional increases in Gammaproteobacteria in IT-operated animals. Furthermore, IT surgery decreased all three sub-arms of ER stress signaling in liver, adipose and pancreas tissues. Amelioration of ER stress coincided with improved insulin signaling and preservation of β-cell mass in IT-operated animals. Incubation of hepatocyte, adipocyte and β-cell lines with cholic acid decreased ER stress. These results suggest that postoperative increases in circulating cholic acid concentration contribute to improvements in glucose homeostasis after IT surgery by ameliorating ER stress.

Original languageEnglish (US)
Pages (from-to)443-456
Number of pages14
JournalDMM Disease Models and Mechanisms
Volume6
Issue number2
DOIs
StatePublished - Mar 2013

Fingerprint

Endoplasmic Reticulum Stress
Bile Acids and Salts
Surgery
Rats
Animals
Cholic Acid
Glucose
Glucose Tolerance Test
Homeostasis
Fats
Body Weight
Tissue
Gammaproteobacteria
Glucagon-Like Peptide 1
Bariatric Surgery
Medical problems
Energy Intake
Lipid Metabolism
Cell culture
Adipocytes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine (miscellaneous)
  • Immunology and Microbiology (miscellaneous)
  • Neuroscience (miscellaneous)

Cite this

Bile-acid-mediated decrease in endoplasmic reticulum stress : A potential contributor to the metabolic benefits of ileal interposition surgery in UCD-T2DM rats. / Cummings, Bethany P.; Bettaieb, Ahmed; Graham, James L.; Kim, Jaehyoung; Ma, Fangrui; Shibata, Noreene; Stanhope, Kimber; Giulivi, Cecilia R; Hansen, Frederik; Jelsing, Jacob; Vrang, Niels; Kowala, Mark; Chouinard, Michael L.; Haj, Fawaz; Havel, Peter J.

In: DMM Disease Models and Mechanisms, Vol. 6, No. 2, 03.2013, p. 443-456.

Research output: Contribution to journalArticle

Cummings, BP, Bettaieb, A, Graham, JL, Kim, J, Ma, F, Shibata, N, Stanhope, K, Giulivi, CR, Hansen, F, Jelsing, J, Vrang, N, Kowala, M, Chouinard, ML, Haj, F & Havel, PJ 2013, 'Bile-acid-mediated decrease in endoplasmic reticulum stress: A potential contributor to the metabolic benefits of ileal interposition surgery in UCD-T2DM rats', DMM Disease Models and Mechanisms, vol. 6, no. 2, pp. 443-456. https://doi.org/10.1242/dmm.010421
Cummings, Bethany P. ; Bettaieb, Ahmed ; Graham, James L. ; Kim, Jaehyoung ; Ma, Fangrui ; Shibata, Noreene ; Stanhope, Kimber ; Giulivi, Cecilia R ; Hansen, Frederik ; Jelsing, Jacob ; Vrang, Niels ; Kowala, Mark ; Chouinard, Michael L. ; Haj, Fawaz ; Havel, Peter J. / Bile-acid-mediated decrease in endoplasmic reticulum stress : A potential contributor to the metabolic benefits of ileal interposition surgery in UCD-T2DM rats. In: DMM Disease Models and Mechanisms. 2013 ; Vol. 6, No. 2. pp. 443-456.
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