Bile acid fractionations by high-performance liquid chromatography in equine liver disease

J. J. Kaneko, W. G. Rudolph, Dennis W Wilson, Gary Carlson

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Serum bile acids were fractionated by high-performance liquid chromatography (HPLC) in 13 control and 8 cases of liver disease in horses. The severity and type of liver injury was determined by histopathological examination of biopsy and/or necropsy specimens. The total serum bile acids (tSBA) were determined in these horses by an enzymatic method (SBA-EA) and by summation of the bile acids (SBA-LC) as fractionated by the HPLC. The SBA-LC were generally higher than the SBA-EA in both the controls and liver disease and they did not parallel each other. The primary bile acids, total cholates and total chenodeoxycholates accounted for most of the tSBA increases in liver disease. There was a shift in profile from taurocholate to free (unconjugated) cholate in direct relation to the severity of the liver injury. Among the secondary bile acids, total deoxycholates and total taurodeoxycholates increased at random. The pattern of the SBA profile in relation to the severity of the liver disease suggested that hepatocellular excretion is the most sensitive step in the enterohepatic circulation of the bile acids.

Original languageEnglish (US)
Pages (from-to)161-172
Number of pages12
JournalVeterinary Research Communications
Volume16
Issue number3
DOIs
StatePublished - May 1992

Keywords

  • bile acids
  • horses
  • liquid chromatography
  • liver disease

ASJC Scopus subject areas

  • veterinary(all)

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