Bilayered vascular graft derived from human induced pluripotent stem cells with biomimetic structure and function

Karina H. Nakayama, Prajakta A. Joshi, Edwina S. Lai, Prachi Gujar, Lydia M. Joubert, Bertha Chen, Ngan F. Huang

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Background: We developed an aligned bi-layered vascular graft derived from human induced pluripotent stem cells (iPSCs) that recapitulates the cellular composition, orientation, and anti-inflammatory function of blood vessels. Materials & methods: The luminal layer consisted of longitudinal-aligned nanofibrillar collagen containing primary endothelial cells (ECs) or iPSC-derived ECs (iPSC-ECs). The outer layer contained circumferentially oriented nanofibrillar collagen with primary smooth muscle cells (SMCs) or iPSC-derived SMCs(iPSC-SMCs). Results: On the aligned scaffolds, cells organized F-actin assembly within 8° from the direction of nanofibrils. When compared to randomly-oriented scaffolds, EC-seeded aligned scaffolds had significant reduced inflammatory response, based on adhesivity to monocytes. Conclusion: This study highlights the importance of anisotropic scaffolds in directing cell form and function, and has therapeutic significance as physiologically relevant blood vessels.

Original languageEnglish (US)
Pages (from-to)745-755
Number of pages11
JournalRegenerative Medicine
Volume10
Issue number6
DOIs
StatePublished - Sep 1 2015
Externally publishedYes

Keywords

  • collagen
  • endothelial
  • extracellular matrix
  • iPSC
  • nanofibrillar
  • scaffolds
  • smooth muscle
  • stem cells
  • tissue engineering
  • vascular graft

ASJC Scopus subject areas

  • Biomedical Engineering
  • Embryology

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  • Cite this

    Nakayama, K. H., Joshi, P. A., Lai, E. S., Gujar, P., Joubert, L. M., Chen, B., & Huang, N. F. (2015). Bilayered vascular graft derived from human induced pluripotent stem cells with biomimetic structure and function. Regenerative Medicine, 10(6), 745-755. https://doi.org/10.2217/rme.15.45