Bilayered vascular graft derived from human induced pluripotent stem cells with biomimetic structure and function

Karina H. Nakayama, Prajakta A. Joshi, Edwina S. Lai, Prachi Gujar, Lydia M. Joubert, Bertha Chen, Ngan F. Huang

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Background: We developed an aligned bi-layered vascular graft derived from human induced pluripotent stem cells (iPSCs) that recapitulates the cellular composition, orientation, and anti-inflammatory function of blood vessels. Materials & methods: The luminal layer consisted of longitudinal-aligned nanofibrillar collagen containing primary endothelial cells (ECs) or iPSC-derived ECs (iPSC-ECs). The outer layer contained circumferentially oriented nanofibrillar collagen with primary smooth muscle cells (SMCs) or iPSC-derived SMCs(iPSC-SMCs). Results: On the aligned scaffolds, cells organized F-actin assembly within 8° from the direction of nanofibrils. When compared to randomly-oriented scaffolds, EC-seeded aligned scaffolds had significant reduced inflammatory response, based on adhesivity to monocytes. Conclusion: This study highlights the importance of anisotropic scaffolds in directing cell form and function, and has therapeutic significance as physiologically relevant blood vessels.

Original languageEnglish (US)
Pages (from-to)745-755
Number of pages11
JournalRegenerative Medicine
Volume10
Issue number6
DOIs
StatePublished - Sep 1 2015
Externally publishedYes

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Induced Pluripotent Stem Cells
Biomimetics
Stem cells
Grafts
Scaffolds
Blood Vessels
Endothelial cells
Endothelial Cells
Transplants
Smooth Muscle Myocytes
Blood vessels
Collagen
Muscle
Forms (concrete)
Actins
Monocytes
Anti-Inflammatory Agents
Cells
Chemical analysis
Therapeutics

Keywords

  • collagen
  • endothelial
  • extracellular matrix
  • iPSC
  • nanofibrillar
  • scaffolds
  • smooth muscle
  • stem cells
  • tissue engineering
  • vascular graft

ASJC Scopus subject areas

  • Biomedical Engineering
  • Embryology

Cite this

Nakayama, K. H., Joshi, P. A., Lai, E. S., Gujar, P., Joubert, L. M., Chen, B., & Huang, N. F. (2015). Bilayered vascular graft derived from human induced pluripotent stem cells with biomimetic structure and function. Regenerative Medicine, 10(6), 745-755. https://doi.org/10.2217/rme.15.45

Bilayered vascular graft derived from human induced pluripotent stem cells with biomimetic structure and function. / Nakayama, Karina H.; Joshi, Prajakta A.; Lai, Edwina S.; Gujar, Prachi; Joubert, Lydia M.; Chen, Bertha; Huang, Ngan F.

In: Regenerative Medicine, Vol. 10, No. 6, 01.09.2015, p. 745-755.

Research output: Contribution to journalArticle

Nakayama, KH, Joshi, PA, Lai, ES, Gujar, P, Joubert, LM, Chen, B & Huang, NF 2015, 'Bilayered vascular graft derived from human induced pluripotent stem cells with biomimetic structure and function', Regenerative Medicine, vol. 10, no. 6, pp. 745-755. https://doi.org/10.2217/rme.15.45
Nakayama, Karina H. ; Joshi, Prajakta A. ; Lai, Edwina S. ; Gujar, Prachi ; Joubert, Lydia M. ; Chen, Bertha ; Huang, Ngan F. / Bilayered vascular graft derived from human induced pluripotent stem cells with biomimetic structure and function. In: Regenerative Medicine. 2015 ; Vol. 10, No. 6. pp. 745-755.
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