Bifidobacterium longum subsp. infantis ATCC 15697 α-fucosidases are active on fucosylated human milk oligosaccharides

David A. Sela, Daniel Garrido, Larry Lerno, Shuai Wu, Kemin Tan, Hyun Ju Eom, Andrzej Joachimiak, Carlito B Lebrilla, David A. Mills

Research output: Contribution to journalArticle

106 Scopus citations

Abstract

Bifidobacterium longum subsp. infantis ATCC 15697 utilizes several small-mass neutral human milk oligosaccharides (HMOs), several of which are fucosylated. Whereas previous studies focused on endpoint consumption, a temporal glycan consumption profile revealed a time-dependent effect. Specifically, among preferred HMOs, tetraose was favored early in fermentation, with other oligosaccharides consumed slightly later. In order to utilize fucosylated oligosaccharides, ATCC 15697 possesses several fucosidases, implicating GH29 and GH95α-L-fucosidases in a gene cluster dedicated to HMO metabolism. Evaluation of the biochemical kinetics demonstrated that ATCC 15697 expresses three fucosidases with a high turnover rate. Moreover, several ATCC 15697 fucosidases are active on the linkages inherent to the HMO molecule. Finally, the HMO cluster GH29α-L-fucosidase possesses a crystal structure that is similar to previously characterized fucosidases.

Original languageEnglish (US)
Pages (from-to)795-803
Number of pages9
JournalApplied and Environmental Microbiology
Volume78
Issue number3
DOIs
StatePublished - Feb 2012

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Food Science
  • Biotechnology
  • Ecology

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