Bifidobacterium longum subsp. infantis ATCC 15697 α-fucosidases are active on fucosylated human milk oligosaccharides

David A. Sela, Daniel Garrido, Larry Lerno, Shuai Wu, Kemin Tan, Hyun Ju Eom, Andrzej Joachimiak, Carlito B Lebrilla, David A. Mills

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Abstract

Bifidobacterium longum subsp. infantis ATCC 15697 utilizes several small-mass neutral human milk oligosaccharides (HMOs), several of which are fucosylated. Whereas previous studies focused on endpoint consumption, a temporal glycan consumption profile revealed a time-dependent effect. Specifically, among preferred HMOs, tetraose was favored early in fermentation, with other oligosaccharides consumed slightly later. In order to utilize fucosylated oligosaccharides, ATCC 15697 possesses several fucosidases, implicating GH29 and GH95α-L-fucosidases in a gene cluster dedicated to HMO metabolism. Evaluation of the biochemical kinetics demonstrated that ATCC 15697 expresses three fucosidases with a high turnover rate. Moreover, several ATCC 15697 fucosidases are active on the linkages inherent to the HMO molecule. Finally, the HMO cluster GH29α-L-fucosidase possesses a crystal structure that is similar to previously characterized fucosidases.

Original languageEnglish (US)
Pages (from-to)795-803
Number of pages9
JournalApplied and Environmental Microbiology
Volume78
Issue number3
DOIs
StatePublished - Feb 2012

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Bifidobacterium longum subsp. infantis
alpha-L-Fucosidase
Human Milk
breast milk
Oligosaccharides
oligosaccharides
milk
crystal structure
fermentation
turnover
metabolism
Multigene Family
Bifidobacterium longum
endpoints
multigene family
Fermentation
Polysaccharides
linkage (genetics)
kinetics
gene

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Food Science
  • Biotechnology
  • Ecology

Cite this

Bifidobacterium longum subsp. infantis ATCC 15697 α-fucosidases are active on fucosylated human milk oligosaccharides. / Sela, David A.; Garrido, Daniel; Lerno, Larry; Wu, Shuai; Tan, Kemin; Eom, Hyun Ju; Joachimiak, Andrzej; Lebrilla, Carlito B; Mills, David A.

In: Applied and Environmental Microbiology, Vol. 78, No. 3, 02.2012, p. 795-803.

Research output: Contribution to journalArticle

Sela, DA, Garrido, D, Lerno, L, Wu, S, Tan, K, Eom, HJ, Joachimiak, A, Lebrilla, CB & Mills, DA 2012, 'Bifidobacterium longum subsp. infantis ATCC 15697 α-fucosidases are active on fucosylated human milk oligosaccharides', Applied and Environmental Microbiology, vol. 78, no. 3, pp. 795-803. https://doi.org/10.1128/AEM.06762-11
Sela, David A. ; Garrido, Daniel ; Lerno, Larry ; Wu, Shuai ; Tan, Kemin ; Eom, Hyun Ju ; Joachimiak, Andrzej ; Lebrilla, Carlito B ; Mills, David A. / Bifidobacterium longum subsp. infantis ATCC 15697 α-fucosidases are active on fucosylated human milk oligosaccharides. In: Applied and Environmental Microbiology. 2012 ; Vol. 78, No. 3. pp. 795-803.
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