Abstract
The L-arginine acts stereoselectively on the Pc1.LSU nuclear group I intron of Pneumocystis carinii, competitively inhibiting the first step of the splicing reaction and stimulating the second step. A number of arginine-related compounds are more potent than L-arginine as stimulators and inhibitors. The most potent peptides tested are 10,000 times as effective as L-arginine in inhibiting ribozyme activity, and nearly 400 times as effective as stimulators. This phenomenon indicates that ribozymes, like protein enzymes, can interact with non-substrate low molecular weight compounds, and that non-nucleic acid agents might be developed as drugs acting on RNA targets.
| Original language | English (US) |
|---|---|
| Journal | The Journal of eukaryotic microbiology |
| Volume | 41 |
| Issue number | 5 |
| State | Published - Sep 1994 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Agricultural and Biological Sciences (miscellaneous)
- Applied Microbiology and Biotechnology
- Microbiology
Cite this
Bidirectional effectors of a group I intron ribozyme from Pneumocystis carinii. / Liu, Y.; Leibowitz, Michael J.
In: The Journal of eukaryotic microbiology, Vol. 41, No. 5, 09.1994.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Bidirectional effectors of a group I intron ribozyme from Pneumocystis carinii.
AU - Liu, Y.
AU - Leibowitz, Michael J
PY - 1994/9
Y1 - 1994/9
N2 - The L-arginine acts stereoselectively on the Pc1.LSU nuclear group I intron of Pneumocystis carinii, competitively inhibiting the first step of the splicing reaction and stimulating the second step. A number of arginine-related compounds are more potent than L-arginine as stimulators and inhibitors. The most potent peptides tested are 10,000 times as effective as L-arginine in inhibiting ribozyme activity, and nearly 400 times as effective as stimulators. This phenomenon indicates that ribozymes, like protein enzymes, can interact with non-substrate low molecular weight compounds, and that non-nucleic acid agents might be developed as drugs acting on RNA targets.
AB - The L-arginine acts stereoselectively on the Pc1.LSU nuclear group I intron of Pneumocystis carinii, competitively inhibiting the first step of the splicing reaction and stimulating the second step. A number of arginine-related compounds are more potent than L-arginine as stimulators and inhibitors. The most potent peptides tested are 10,000 times as effective as L-arginine in inhibiting ribozyme activity, and nearly 400 times as effective as stimulators. This phenomenon indicates that ribozymes, like protein enzymes, can interact with non-substrate low molecular weight compounds, and that non-nucleic acid agents might be developed as drugs acting on RNA targets.
UR - http://www.scopus.com/inward/record.url?scp=19244376922&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=19244376922&partnerID=8YFLogxK
M3 - Article
VL - 41
JO - Journal of Eukaryotic Microbiology
JF - Journal of Eukaryotic Microbiology
SN - 1066-5234
IS - 5
ER -