Abstract
Much scientific effort has been directed towards elucidating the complexities of cell-mediated immune responses to HIV-1 (reviewed in [1,2]). These studies have attempted to explain the immune system's ultimate failure to contain viral replication, leading to development of AIDS disease, and to identify immune responses that will be useful in developing immunomodulatory therapies and novel vaccine strategies. Although many of the complex interactions involved in AIDS pathogenesis remain unsolved, great progress has been made in characterizing the kinetics, specificity and functional dynamics of HIV-1-specific T cell responses. These investigations have come at a time when advances in virology, cellular immunology and molecular biology have converged to provide a variety of methodological approaches not available at the onset of the AIDS pandemic. Application of these tools to other infectious diseases and immunopathological conditions will provide a fertile area of research for future years. This review focuses on recent developments in the assessment of HIV-1-specific T cell responses in peripheral blood and tissues, with a particular emphasis on flow cytometry-based approaches.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 172-182 |
| Number of pages | 11 |
| Journal | Clinical and Experimental Immunology |
| Volume | 130 |
| Issue number | 2 |
| DOIs | |
| State | Published - 2002 |
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ASJC Scopus subject areas
- Immunology
Cite this
Beyond 51Cr release : New methods for assessing HIV-1-specific CD8+ T cell responses in peripheral blood and mucosal tissues. / Shacklett, Barbara.
In: Clinical and Experimental Immunology, Vol. 130, No. 2, 2002, p. 172-182.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Beyond 51Cr release
T2 - New methods for assessing HIV-1-specific CD8+ T cell responses in peripheral blood and mucosal tissues
AU - Shacklett, Barbara
PY - 2002
Y1 - 2002
N2 - Much scientific effort has been directed towards elucidating the complexities of cell-mediated immune responses to HIV-1 (reviewed in [1,2]). These studies have attempted to explain the immune system's ultimate failure to contain viral replication, leading to development of AIDS disease, and to identify immune responses that will be useful in developing immunomodulatory therapies and novel vaccine strategies. Although many of the complex interactions involved in AIDS pathogenesis remain unsolved, great progress has been made in characterizing the kinetics, specificity and functional dynamics of HIV-1-specific T cell responses. These investigations have come at a time when advances in virology, cellular immunology and molecular biology have converged to provide a variety of methodological approaches not available at the onset of the AIDS pandemic. Application of these tools to other infectious diseases and immunopathological conditions will provide a fertile area of research for future years. This review focuses on recent developments in the assessment of HIV-1-specific T cell responses in peripheral blood and tissues, with a particular emphasis on flow cytometry-based approaches.
AB - Much scientific effort has been directed towards elucidating the complexities of cell-mediated immune responses to HIV-1 (reviewed in [1,2]). These studies have attempted to explain the immune system's ultimate failure to contain viral replication, leading to development of AIDS disease, and to identify immune responses that will be useful in developing immunomodulatory therapies and novel vaccine strategies. Although many of the complex interactions involved in AIDS pathogenesis remain unsolved, great progress has been made in characterizing the kinetics, specificity and functional dynamics of HIV-1-specific T cell responses. These investigations have come at a time when advances in virology, cellular immunology and molecular biology have converged to provide a variety of methodological approaches not available at the onset of the AIDS pandemic. Application of these tools to other infectious diseases and immunopathological conditions will provide a fertile area of research for future years. This review focuses on recent developments in the assessment of HIV-1-specific T cell responses in peripheral blood and tissues, with a particular emphasis on flow cytometry-based approaches.
UR - http://www.scopus.com/inward/record.url?scp=0036025337&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036025337&partnerID=8YFLogxK
U2 - 10.1046/j.1365-2249.2002.01981.x
DO - 10.1046/j.1365-2249.2002.01981.x
M3 - Article
C2 - 12390303
AN - SCOPUS:0036025337
VL - 130
SP - 172
EP - 182
JO - Clinical and Experimental Immunology
JF - Clinical and Experimental Immunology
SN - 0009-9104
IS - 2
ER -