Beyond 51Cr release: New methods for assessing HIV-1-specific CD8+ T cell responses in peripheral blood and mucosal tissues

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Much scientific effort has been directed towards elucidating the complexities of cell-mediated immune responses to HIV-1 (reviewed in [1,2]). These studies have attempted to explain the immune system's ultimate failure to contain viral replication, leading to development of AIDS disease, and to identify immune responses that will be useful in developing immunomodulatory therapies and novel vaccine strategies. Although many of the complex interactions involved in AIDS pathogenesis remain unsolved, great progress has been made in characterizing the kinetics, specificity and functional dynamics of HIV-1-specific T cell responses. These investigations have come at a time when advances in virology, cellular immunology and molecular biology have converged to provide a variety of methodological approaches not available at the onset of the AIDS pandemic. Application of these tools to other infectious diseases and immunopathological conditions will provide a fertile area of research for future years. This review focuses on recent developments in the assessment of HIV-1-specific T cell responses in peripheral blood and tissues, with a particular emphasis on flow cytometry-based approaches.

Original languageEnglish (US)
Pages (from-to)172-182
Number of pages11
JournalClinical and Experimental Immunology
Volume130
Issue number2
DOIs
StatePublished - 2002

Fingerprint

HIV-1
Mucous Membrane
Acquired Immunodeficiency Syndrome
T-Lymphocytes
Virology
Immunomodulation
Pandemics
Allergy and Immunology
Communicable Diseases
Cell Biology
Molecular Biology
Immune System
Flow Cytometry
Vaccines
Research

ASJC Scopus subject areas

  • Immunology

Cite this

@article{99808caf13164367aff86859d3bb0313,
title = "Beyond 51Cr release: New methods for assessing HIV-1-specific CD8+ T cell responses in peripheral blood and mucosal tissues",
abstract = "Much scientific effort has been directed towards elucidating the complexities of cell-mediated immune responses to HIV-1 (reviewed in [1,2]). These studies have attempted to explain the immune system's ultimate failure to contain viral replication, leading to development of AIDS disease, and to identify immune responses that will be useful in developing immunomodulatory therapies and novel vaccine strategies. Although many of the complex interactions involved in AIDS pathogenesis remain unsolved, great progress has been made in characterizing the kinetics, specificity and functional dynamics of HIV-1-specific T cell responses. These investigations have come at a time when advances in virology, cellular immunology and molecular biology have converged to provide a variety of methodological approaches not available at the onset of the AIDS pandemic. Application of these tools to other infectious diseases and immunopathological conditions will provide a fertile area of research for future years. This review focuses on recent developments in the assessment of HIV-1-specific T cell responses in peripheral blood and tissues, with a particular emphasis on flow cytometry-based approaches.",
author = "Barbara Shacklett",
year = "2002",
doi = "10.1046/j.1365-2249.2002.01981.x",
language = "English (US)",
volume = "130",
pages = "172--182",
journal = "Clinical and Experimental Immunology",
issn = "0009-9104",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Beyond 51Cr release

T2 - New methods for assessing HIV-1-specific CD8+ T cell responses in peripheral blood and mucosal tissues

AU - Shacklett, Barbara

PY - 2002

Y1 - 2002

N2 - Much scientific effort has been directed towards elucidating the complexities of cell-mediated immune responses to HIV-1 (reviewed in [1,2]). These studies have attempted to explain the immune system's ultimate failure to contain viral replication, leading to development of AIDS disease, and to identify immune responses that will be useful in developing immunomodulatory therapies and novel vaccine strategies. Although many of the complex interactions involved in AIDS pathogenesis remain unsolved, great progress has been made in characterizing the kinetics, specificity and functional dynamics of HIV-1-specific T cell responses. These investigations have come at a time when advances in virology, cellular immunology and molecular biology have converged to provide a variety of methodological approaches not available at the onset of the AIDS pandemic. Application of these tools to other infectious diseases and immunopathological conditions will provide a fertile area of research for future years. This review focuses on recent developments in the assessment of HIV-1-specific T cell responses in peripheral blood and tissues, with a particular emphasis on flow cytometry-based approaches.

AB - Much scientific effort has been directed towards elucidating the complexities of cell-mediated immune responses to HIV-1 (reviewed in [1,2]). These studies have attempted to explain the immune system's ultimate failure to contain viral replication, leading to development of AIDS disease, and to identify immune responses that will be useful in developing immunomodulatory therapies and novel vaccine strategies. Although many of the complex interactions involved in AIDS pathogenesis remain unsolved, great progress has been made in characterizing the kinetics, specificity and functional dynamics of HIV-1-specific T cell responses. These investigations have come at a time when advances in virology, cellular immunology and molecular biology have converged to provide a variety of methodological approaches not available at the onset of the AIDS pandemic. Application of these tools to other infectious diseases and immunopathological conditions will provide a fertile area of research for future years. This review focuses on recent developments in the assessment of HIV-1-specific T cell responses in peripheral blood and tissues, with a particular emphasis on flow cytometry-based approaches.

UR - http://www.scopus.com/inward/record.url?scp=0036025337&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036025337&partnerID=8YFLogxK

U2 - 10.1046/j.1365-2249.2002.01981.x

DO - 10.1046/j.1365-2249.2002.01981.x

M3 - Article

C2 - 12390303

AN - SCOPUS:0036025337

VL - 130

SP - 172

EP - 182

JO - Clinical and Experimental Immunology

JF - Clinical and Experimental Immunology

SN - 0009-9104

IS - 2

ER -