Best practices for performance of real-time PCR assays in veterinary diagnostic laboratories

Kathy L. Kurth, Donna M. Mulrooney, Susanne Hinkley, Mary Lea Killian, Janice C. Pedersen, Mangkey A. Bounpheng, Roman Pogranichniy, Steve Bolin, Roger Maes, Rebecca L. Tallmadge, Laura B. Goodman, Beate M. Crossley

Research output: Contribution to journalArticlepeer-review

Abstract

The exquisite sensitivity of in vitro amplification assays such as real-time polymerase chain reaction (rtPCR) requires the establishment of thorough and robust laboratory practices. To this end, an American Association of Veterinary Laboratory Diagnosticians (AAVLD) committee of subject matter experts was convened to develop a set of best practices for performance of nucleic acid amplification assays. Consensus advice for the performance of preanalytical, analytical, and postanalytical steps is presented here, along with a review of supporting literature.

Original languageEnglish (US)
JournalJournal of Veterinary Diagnostic Investigation
DOIs
StateAccepted/In press - 2020

Keywords

  • AAVLD
  • practice guidelines
  • qPCR
  • real-time PCR

ASJC Scopus subject areas

  • veterinary(all)

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