Best practice BioBanking of human heart tissue

Sean Lal, Amy Li, David Allen, Paul D. Allen, Paul Bannon, Tim Cartmill, Roger Cooke, Alan Farnsworth, Anne Keogh, Cristobal dos Remedios

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

This review provides a guide to researchers who wish to establish a biobank. It also gives practical advice to investigators seeking access to samples of healthy or diseased human hearts. We begin with a brief history of the Sydney Heart Bank (SHB) from when it began in 1989, including the pivotal role played by the late Victor Chang. We discuss our standard operating procedures for tissue collection which include cryopreservation and the quality assurance needed to maintain the long-term molecular and cellular integrity of the samples. The SHB now contains about 16,000 heart samples derived from over 450 patients who underwent isotopic heart transplant procedures and from over 100 healthy organ donors. These enable us to provide samples from a wide range of categories of heart failure. So far, we have delivered heart samples to more than 50 laboratories over two decades, and we answer their most frequently asked questions. Other SHB services include the development of tissue microarrays (TMA). These enable end users to perform preliminary examinations of the expression and localisation of target molecules in diseased or aging donor hearts, all in a single section of the TMA. Finally, the processes involved in managing tissue requests from external users and logistics considerations for the shipment of human tissue are discussed in detail.

Original languageEnglish (US)
Pages (from-to)399-406
Number of pages8
JournalBiophysical Reviews
Volume7
Issue number4
DOIs
StatePublished - Dec 1 2015

Keywords

  • BioBanking
  • Healthy donor tissue
  • Heart failure
  • Human heart tissue
  • Sydney Heart Bank

ASJC Scopus subject areas

  • Biophysics
  • Molecular Biology
  • Structural Biology

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    Lal, S., Li, A., Allen, D., Allen, P. D., Bannon, P., Cartmill, T., Cooke, R., Farnsworth, A., Keogh, A., & dos Remedios, C. (2015). Best practice BioBanking of human heart tissue. Biophysical Reviews, 7(4), 399-406. https://doi.org/10.1007/s12551-015-0182-6