Benzyl derivative facilitation of transcription in Escherichia coli at the ara and lac operon promoters: Metabolite gene regulation (MGR)

Ellis L. Kline, Robert W. West, Barbara S. Ink, Priscilla M. Kline, Raymond L. Rodriguez

Research output: Contribution to journalArticle

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Abstract

A number of benzyl derivatives have been tested for their ability to induce the expression of the araBAD operon in an Escherichia coli K-12 strain. Those derivatives shown to be stimulatory include: benzoic acid (BA), paramino benzoic acid (PABA), para-hydroxy benzoic acid (PHBA), ortho-amino benzoic acid (OABA), 3-hydroxy-4-methoxy phenylethylamine (MTA), and 4-hydroxy-3-methoxyphenol acetic acid (HVA). The araC gene product was necessary to facilitate the induction. To further characterize if the inductive effect was mediated at the level of transcription, an araBAD-tetracycline resistant (Tcr) operon fusion plasmid (pAP-B) was employed. Benzyl derivatives which induce expression of the araBAD operon in situ also induced a Tcr phenotype with pAP-B. Both indole acetic acid (IAA) and imidazole (IM), which were previously shown to circumvent the necessity for cAMP in the induction of the araBAD operon, also induced a Tcr phenotype with pAP-B. Induction of lac or othe cAMP responding operons with the inducing molecules at the chromosomal level was not detectable when assessed by carbon utilization. However, a lacZYA-Tcr operon fusion plasmid (pLPI) did respond to IAA and several of the inducing benzyl derivatives. Catabolite repression of chromosomal araBAD expression was reversed when the exogenous concentration of OABA was elevated. Similar effects on the Tcr phenotypes conferred by pAP-B and pLP1 were observed when OABA or several other inducing benzyl derivatives were present exogenously.

Original languageEnglish (US)
Pages (from-to)340-348
Number of pages9
JournalMGG Molecular & General Genetics
Volume193
Issue number2
DOIs
StatePublished - Feb 1984

Fingerprint

Lac Operon
Operon
Benzoic Acid
Tetracycline
Escherichia coli
Genes
Phenotype
araC Genes
Plasmids
Vanillic Acid
Catabolite Repression
Phenethylamines
Acetic Acid
Carbon

ASJC Scopus subject areas

  • Genetics

Cite this

Benzyl derivative facilitation of transcription in Escherichia coli at the ara and lac operon promoters : Metabolite gene regulation (MGR). / Kline, Ellis L.; West, Robert W.; Ink, Barbara S.; Kline, Priscilla M.; Rodriguez, Raymond L.

In: MGG Molecular & General Genetics, Vol. 193, No. 2, 02.1984, p. 340-348.

Research output: Contribution to journalArticle

Kline, Ellis L. ; West, Robert W. ; Ink, Barbara S. ; Kline, Priscilla M. ; Rodriguez, Raymond L. / Benzyl derivative facilitation of transcription in Escherichia coli at the ara and lac operon promoters : Metabolite gene regulation (MGR). In: MGG Molecular & General Genetics. 1984 ; Vol. 193, No. 2. pp. 340-348.
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