Behavioral investigation of the coexistence of substance P, corticotropin releasing factor, and acetylcholinesterase in lateral dorsal tegmental neurons projecting to the medial frontal cortex of the rat

Jacqueline Crawley, John A. Olschowka, Debra I. Diz, David M. Jacobowitz

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Colocalization of substance P (SP), corticotropin releasing factor (CRF), and acetylcholinesterase (AChE) was detected by retrograde tracing and immunocytochemical staining in the nucleus tegmentalis dorsalis lateralis (ntdl) projecting to the medial frontal cortex (MFC), septum, and thalamus of the rat. The histochemical results suggest that SP and CRF coexist within a subpopulation of ntdl cholinergic neurons that project to a number of forebrain regions including the MFC. Behavioral studies of the effects of SP, CRF, and the cholinergic agonist, carbachol, employed microinjections into the MFC of rats. SP and CRF did not elicit any behavioral effects when administered alone. Carbachol (1-5 μg/side) produced a stereotyped motor behavior, consisting of rapid forepaw treading while in an upright posture, resembling "boxing." SP (1 μg/side) increased carbachol-induced "boxing." CRF (1-10 ng/side) decreased carbachol-induced "boxing." One possible functional significance of the coexistence of SP, CRF, and acetylcholinesterase, in neurons projecting to the medial frontal cortex in rats, appears to be a modulatory potentiation of cholinergic response by SP, and a modulatory inhibition of the cholinergic response by CRF.

Original languageEnglish (US)
Pages (from-to)891-901
Number of pages11
JournalPeptides
Volume6
Issue number5
DOIs
StatePublished - 1985
Externally publishedYes

Fingerprint

Corticotropin-Releasing Hormone
Frontal Lobe
Acetylcholinesterase
Substance P
Neurons
Rats
Boxing
Carbachol
Cholinergic Agents
Stereotyped Behavior
Cholinergic Agonists
Cholinergic Neurons
Microinjections
Prosencephalon
Posture
Thalamus
Staining and Labeling

Keywords

  • Acetylcholine
  • Acetylcholinesterase
  • Behavior
  • Coexistence
  • Corticotropin releasing factor
  • Immunocytochemistry
  • Medial frontal cortex
  • Nucleus tegmentalis dorsalis lateralis
  • Substance P

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

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title = "Behavioral investigation of the coexistence of substance P, corticotropin releasing factor, and acetylcholinesterase in lateral dorsal tegmental neurons projecting to the medial frontal cortex of the rat",
abstract = "Colocalization of substance P (SP), corticotropin releasing factor (CRF), and acetylcholinesterase (AChE) was detected by retrograde tracing and immunocytochemical staining in the nucleus tegmentalis dorsalis lateralis (ntdl) projecting to the medial frontal cortex (MFC), septum, and thalamus of the rat. The histochemical results suggest that SP and CRF coexist within a subpopulation of ntdl cholinergic neurons that project to a number of forebrain regions including the MFC. Behavioral studies of the effects of SP, CRF, and the cholinergic agonist, carbachol, employed microinjections into the MFC of rats. SP and CRF did not elicit any behavioral effects when administered alone. Carbachol (1-5 μg/side) produced a stereotyped motor behavior, consisting of rapid forepaw treading while in an upright posture, resembling {"}boxing.{"} SP (1 μg/side) increased carbachol-induced {"}boxing.{"} CRF (1-10 ng/side) decreased carbachol-induced {"}boxing.{"} One possible functional significance of the coexistence of SP, CRF, and acetylcholinesterase, in neurons projecting to the medial frontal cortex in rats, appears to be a modulatory potentiation of cholinergic response by SP, and a modulatory inhibition of the cholinergic response by CRF.",
keywords = "Acetylcholine, Acetylcholinesterase, Behavior, Coexistence, Corticotropin releasing factor, Immunocytochemistry, Medial frontal cortex, Nucleus tegmentalis dorsalis lateralis, Substance P",
author = "Jacqueline Crawley and Olschowka, {John A.} and Diz, {Debra I.} and Jacobowitz, {David M.}",
year = "1985",
doi = "10.1016/0196-9781(85)90320-1",
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pages = "891--901",
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T1 - Behavioral investigation of the coexistence of substance P, corticotropin releasing factor, and acetylcholinesterase in lateral dorsal tegmental neurons projecting to the medial frontal cortex of the rat

AU - Crawley, Jacqueline

AU - Olschowka, John A.

AU - Diz, Debra I.

AU - Jacobowitz, David M.

PY - 1985

Y1 - 1985

N2 - Colocalization of substance P (SP), corticotropin releasing factor (CRF), and acetylcholinesterase (AChE) was detected by retrograde tracing and immunocytochemical staining in the nucleus tegmentalis dorsalis lateralis (ntdl) projecting to the medial frontal cortex (MFC), septum, and thalamus of the rat. The histochemical results suggest that SP and CRF coexist within a subpopulation of ntdl cholinergic neurons that project to a number of forebrain regions including the MFC. Behavioral studies of the effects of SP, CRF, and the cholinergic agonist, carbachol, employed microinjections into the MFC of rats. SP and CRF did not elicit any behavioral effects when administered alone. Carbachol (1-5 μg/side) produced a stereotyped motor behavior, consisting of rapid forepaw treading while in an upright posture, resembling "boxing." SP (1 μg/side) increased carbachol-induced "boxing." CRF (1-10 ng/side) decreased carbachol-induced "boxing." One possible functional significance of the coexistence of SP, CRF, and acetylcholinesterase, in neurons projecting to the medial frontal cortex in rats, appears to be a modulatory potentiation of cholinergic response by SP, and a modulatory inhibition of the cholinergic response by CRF.

AB - Colocalization of substance P (SP), corticotropin releasing factor (CRF), and acetylcholinesterase (AChE) was detected by retrograde tracing and immunocytochemical staining in the nucleus tegmentalis dorsalis lateralis (ntdl) projecting to the medial frontal cortex (MFC), septum, and thalamus of the rat. The histochemical results suggest that SP and CRF coexist within a subpopulation of ntdl cholinergic neurons that project to a number of forebrain regions including the MFC. Behavioral studies of the effects of SP, CRF, and the cholinergic agonist, carbachol, employed microinjections into the MFC of rats. SP and CRF did not elicit any behavioral effects when administered alone. Carbachol (1-5 μg/side) produced a stereotyped motor behavior, consisting of rapid forepaw treading while in an upright posture, resembling "boxing." SP (1 μg/side) increased carbachol-induced "boxing." CRF (1-10 ng/side) decreased carbachol-induced "boxing." One possible functional significance of the coexistence of SP, CRF, and acetylcholinesterase, in neurons projecting to the medial frontal cortex in rats, appears to be a modulatory potentiation of cholinergic response by SP, and a modulatory inhibition of the cholinergic response by CRF.

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KW - Substance P

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