Behavioral consequences of marginal iron deficiency during development in a murine model

Catherine L. Kwik-Uribe, Mari S. Golub, Carl L Keen

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Marginal iron deficiency is a common nutritional disorder in human populations world-wide; however, the neurobehavioral effects of chronic marginal iron (Fe) intakes during development are poorly characterized in animals. For this reason, we investigated whether mice reared on marginal Fe diets during pre- and postnatal development would experience neurobehavioral deficits. Swiss-Webster mice reared on either control (75 ppm Fe) or marginal iron (12.5 ppm) diets were assessed for changes in behavior on postnatal days 30, 40, and 50 using a neurobehavioral test battery. Because alterations in tissue mineral status can lead to an oxidative stress, markers of both protein (glutamine synthetase) and lipid oxidation (TBARS) were measured. Marginal iron animals exhibited a 20-55% reduction in grip strength. Although both marginal iron males and females demonstrated persistent lowering of body weights, statistical analysis using weight as the covariate demonstrated that the grip strength reductions were independent of body weight changes. This reduction in grip strength occurred in conjunction with a 25-45% lowering of brain iron in the marginal iron animals. Despite dramatic reductions in both brain and liver iron, hematocrits were unaffected by dietary iron reductions. Oxidative stress was indicated by an elevation in noniron-stimulated TBARS in the cerebellum of marginal iron animals. These data suggest that a chronic marginal Fe deficiency during critical periods of growth can result in functional changes in motor development even in the absence of iron deficiency anemia; furthermore, alterations in mineral status and oxidative stress may be mechanisms contributing to these observed changes. Copyright (C) 1999 Elsevier Science Inc.

Original languageEnglish (US)
Pages (from-to)661-672
Number of pages12
JournalNeurotoxicology and Teratology
Volume21
Issue number6
DOIs
StatePublished - Nov 1999

Fingerprint

Iron
Oxidative stress
Hand Strength
Animals
Oxidative Stress
Nutrition
Minerals
Brain
Nutrition Disorders
Dietary Iron
Diet
Glutamate-Ammonia Ligase
Body Weight Changes
Iron-Deficiency Anemias
Hematocrit
Cerebellum
Liver
Statistical methods
Body Weight
Lipids

Keywords

  • Behavior
  • Development
  • Iron
  • Iron deficiency
  • Oxidative stress

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Neuroscience(all)
  • Toxicology

Cite this

Behavioral consequences of marginal iron deficiency during development in a murine model. / Kwik-Uribe, Catherine L.; Golub, Mari S.; Keen, Carl L.

In: Neurotoxicology and Teratology, Vol. 21, No. 6, 11.1999, p. 661-672.

Research output: Contribution to journalArticle

Kwik-Uribe, Catherine L. ; Golub, Mari S. ; Keen, Carl L. / Behavioral consequences of marginal iron deficiency during development in a murine model. In: Neurotoxicology and Teratology. 1999 ; Vol. 21, No. 6. pp. 661-672.
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