Behavioral characterization of two long-lasting adenosine analogs: Sedative properties and interaction with diazepam

Behavioral analysis of the sedative properties of two potent long-lasting adenosine analogs was performed in mice, using a video-tracking computer-assisted animal behavior monitor. Cyclohexyladenosine (CHA) and 2-chloroadenosine significantly decreased number of movements, increased duration of pauses, and decreased the mean exploratory distance per move, beginning at 0.5 mg/kg i.p. Rotorod analysis of motor coordination and hotplate testing for analgesia also showed significant deficits at these low doses. Diazepam-induced increases in exploratory behavior were reversed by adenosine and its analogs, supporting the possibility of benzodiazepine-adenosine interactions in the central nervous system.