Behavioral abnormalities and circuit defects in the basal ganglia of a mouse model of 16p11.2 deletion syndrome

Thomas Portmann, Mu Yang, Rong Mao, Georgia Panagiotakos, Jacob Ellegood, Gul Dolen, Patrick L. Bader, Brad A. Grueter, Carleton Goold, Elaine Fisher, Katherine Clifford, Pavitra Rengarajan, David Kalikhman, Darren Loureiro, Nay L. Saw, Zhou Zhengqui, Michael A. Miller, Jason P. Lerch, R. Mark Henkelman, Mehrdad ShamlooRobert C. Malenka, Jacqueline Crawley, Ricardo E. Dolmetsch

Research output: Contribution to journalArticle

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Abstract

A deletion on human chromosome 16p11.2 is associated with autism spectrum disorders. We deleted the syntenic region on mouse chromosome 7F3. MRI and high-throughput single-cell transcriptomics revealed anatomical and cellular abnormalities, particularly in cortex and striatum of juvenile mutant mice (16p11+/-). We found elevated numbers of striatal medium spiny neurons (MSNs) expressing the dopamine D2 receptor (Drd2+) and fewer dopamine-sensitive (Drd1+) neurons in deep layers of cortex. Electrophysiological recordings of Drd2+ MSN revealed synaptic defects, suggesting abnormal basal ganglia circuitry function in 16p11+/- mice. This is further supported by behavioral experiments showing hyperactivity, circling, and deficits in movement control. Strikingly, 16p11+/- mice showed a complete lack of habituation reminiscent of what is observed in some autistic individuals. Our findings unveil a fundamental role of genes affected by the 16p11.2 deletion in establishing the basal ganglia circuitry and provide insights in the pathophysiology of autism.

Original languageEnglish (US)
Pages (from-to)1077-1092
Number of pages16
JournalCell Reports
Volume7
Issue number4
DOIs
StatePublished - May 22 2014

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Basal Ganglia
Neurons
Chromosomes
Defects
Networks (circuits)
Dopamine D2 Receptors
Corpus Striatum
Magnetic resonance imaging
Dopamine
Human Chromosomes
Autistic Disorder
Genes
Throughput
16p11.2 Deletion Syndrome
Experiments

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Portmann, T., Yang, M., Mao, R., Panagiotakos, G., Ellegood, J., Dolen, G., ... Dolmetsch, R. E. (2014). Behavioral abnormalities and circuit defects in the basal ganglia of a mouse model of 16p11.2 deletion syndrome. Cell Reports, 7(4), 1077-1092. https://doi.org/10.1016/j.celrep.2014.03.036

Behavioral abnormalities and circuit defects in the basal ganglia of a mouse model of 16p11.2 deletion syndrome. / Portmann, Thomas; Yang, Mu; Mao, Rong; Panagiotakos, Georgia; Ellegood, Jacob; Dolen, Gul; Bader, Patrick L.; Grueter, Brad A.; Goold, Carleton; Fisher, Elaine; Clifford, Katherine; Rengarajan, Pavitra; Kalikhman, David; Loureiro, Darren; Saw, Nay L.; Zhengqui, Zhou; Miller, Michael A.; Lerch, Jason P.; Henkelman, R. Mark; Shamloo, Mehrdad; Malenka, Robert C.; Crawley, Jacqueline; Dolmetsch, Ricardo E.

In: Cell Reports, Vol. 7, No. 4, 22.05.2014, p. 1077-1092.

Research output: Contribution to journalArticle

Portmann, T, Yang, M, Mao, R, Panagiotakos, G, Ellegood, J, Dolen, G, Bader, PL, Grueter, BA, Goold, C, Fisher, E, Clifford, K, Rengarajan, P, Kalikhman, D, Loureiro, D, Saw, NL, Zhengqui, Z, Miller, MA, Lerch, JP, Henkelman, RM, Shamloo, M, Malenka, RC, Crawley, J & Dolmetsch, RE 2014, 'Behavioral abnormalities and circuit defects in the basal ganglia of a mouse model of 16p11.2 deletion syndrome', Cell Reports, vol. 7, no. 4, pp. 1077-1092. https://doi.org/10.1016/j.celrep.2014.03.036
Portmann, Thomas ; Yang, Mu ; Mao, Rong ; Panagiotakos, Georgia ; Ellegood, Jacob ; Dolen, Gul ; Bader, Patrick L. ; Grueter, Brad A. ; Goold, Carleton ; Fisher, Elaine ; Clifford, Katherine ; Rengarajan, Pavitra ; Kalikhman, David ; Loureiro, Darren ; Saw, Nay L. ; Zhengqui, Zhou ; Miller, Michael A. ; Lerch, Jason P. ; Henkelman, R. Mark ; Shamloo, Mehrdad ; Malenka, Robert C. ; Crawley, Jacqueline ; Dolmetsch, Ricardo E. / Behavioral abnormalities and circuit defects in the basal ganglia of a mouse model of 16p11.2 deletion syndrome. In: Cell Reports. 2014 ; Vol. 7, No. 4. pp. 1077-1092.
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AU - Bader, Patrick L.

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AU - Shamloo, Mehrdad

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AU - Dolmetsch, Ricardo E.

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