BCR-ABL translocation in a dog with chronic monocytic leukemia

Janice A. Cruz Cardona, Rowan Milner, A. Rick Alleman, Christina Williams, William Vernau, Matthew Breen, Mary Tompkins

Research output: Contribution to journalArticle

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Abstract

A 9-year-old female spayed mixed breed dog was evaluated at the University of Florida Small Animal Hospital for marked leukocytosis with no associated clinical signs. CBC abnormalities included marked leukocytosis (106,000/μL), marked monocytosis (78,000/μL), and the presence of 13% blast cells (13,832/μL), supporting a diagnosis of leukemia. Cytopenias and dysplastic changes in other cell lines were not present. Microscopic examination of bone marrow showed hypercellular uniparticles with a marginal increase in frequency of unclassified blast cells (2%), but was otherwise unremarkable. Flow cytometric immunophenotyping of blood cells determined that leukemic cells were CD45+, CD14+, and CD34-, and based on side scatter and CD45 reactivity the marrow contained 19% monoblasts. By immunocytochemical staining, the leukemic cells in the bone marrow were CD11b+, CD11c+, CD11d+, MHC-II+, MPO+, and CD34-. Fluorescence in situ hybridization (FISH) analysis of peripheral blood leukocytes documented a chromosomal translocation producing a BCR-ABL gene hybrid, similar to the "Philadelphia" chromosome abnormality recognized in human chronic myelogenous leukemia, as well as a phosphatase and tensin homolog (PTEN) gene deletion. Hydroxyurea therapy was attempted, but was ineffective; the dog died 7 months after initial presentation. Clinical and laboratory findings and the protracted course supported a diagnosis of chronic monocytic leukemia (CMoL) and, to our knowledge, this is the first case of CMoL with a BCR-ABL chromosomal abnormalitiy described in dogs. This may have clinical implications for treatment of dogs with chronic leukemias associated with particular genetic mutations. However, more case studies are needed to further characterize this disease.

Original languageEnglish (US)
Pages (from-to)40-47
Number of pages8
JournalVeterinary Clinical Pathology
Volume40
Issue number1
DOIs
StatePublished - Mar 2011

Fingerprint

Myeloid Leukemia
leukemia
Dogs
dogs
Leukocytosis
Leukemia
Monocyte-Macrophage Precursor Cells
Bone Marrow Examination
Animal Hospitals
Philadelphia Chromosome
Immunophenotyping
Genetic Translocation
Hydroxyurea
Gene Deletion
hydroxyurea
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Fluorescence In Situ Hybridization
Phosphoric Monoester Hydrolases
Chromosome Aberrations
Bone Marrow Cells

Keywords

  • FISH
  • Flow cytometry
  • Monocytosis
  • Myeloproliferative disorder

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Cruz Cardona, J. A., Milner, R., Alleman, A. R., Williams, C., Vernau, W., Breen, M., & Tompkins, M. (2011). BCR-ABL translocation in a dog with chronic monocytic leukemia. Veterinary Clinical Pathology, 40(1), 40-47. https://doi.org/10.1111/j.1939-165X.2010.00277.x

BCR-ABL translocation in a dog with chronic monocytic leukemia. / Cruz Cardona, Janice A.; Milner, Rowan; Alleman, A. Rick; Williams, Christina; Vernau, William; Breen, Matthew; Tompkins, Mary.

In: Veterinary Clinical Pathology, Vol. 40, No. 1, 03.2011, p. 40-47.

Research output: Contribution to journalArticle

Cruz Cardona, JA, Milner, R, Alleman, AR, Williams, C, Vernau, W, Breen, M & Tompkins, M 2011, 'BCR-ABL translocation in a dog with chronic monocytic leukemia', Veterinary Clinical Pathology, vol. 40, no. 1, pp. 40-47. https://doi.org/10.1111/j.1939-165X.2010.00277.x
Cruz Cardona, Janice A. ; Milner, Rowan ; Alleman, A. Rick ; Williams, Christina ; Vernau, William ; Breen, Matthew ; Tompkins, Mary. / BCR-ABL translocation in a dog with chronic monocytic leukemia. In: Veterinary Clinical Pathology. 2011 ; Vol. 40, No. 1. pp. 40-47.
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