BCL2 translocation frequency rises with age in humans

Yafei Liu, Antonio M. Hernandez, Darryl Shibata, Gino A Cortopassi

Research output: Contribution to journalArticle

309 Citations (Scopus)

Abstract

The background frequency of t(14;18) (q32;q21) chromosomal translocations at the locus associated with B-cell leukemia/lymphoma-2 (BCL2) was determined from a survey of the peripheral blood lymphocytes (PBLs) of 53 living individuals and from tissues of 31 autopsies by using a nested PCR assay. The translocation was detected in 55% of PBLs and 35% of autopsied spleens with a frequency of between less than 1 to 853 translocations per million cells. Translocations copurified with B lymphocytes. The frequency of translocations significantly increased with age in PBLs and spleens, as does human risk for lymphoma. Average translocation frequency was more than 40 times greater in the spleen and 13 times greater in the peripheral blood in the oldest individuals (61 yr and older) compared with the youngest individuals (20 yr or younger). Particular t(14;18)-bearing clones persisted over a period of 5 months in two individuals. These findings demonstrate that clones harboring the oncogenic t(14;18) chromosomal translocation are commonly present in normal humans, that such clones are long-lived, and that they rise in frequency with age. A multihit model of lymphomagenesis involving t(14;18) translocation followed by antigen stimulation is proposed.

Original languageEnglish (US)
Pages (from-to)8910-8914
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume91
Issue number19
DOIs
StatePublished - Sep 13 1994
Externally publishedYes

Fingerprint

B-Cell Leukemia
B-Cell Lymphoma
Genetic Translocation
Spleen
Clone Cells
Lymphocytes
Autopsy
Lymphoma
B-Lymphocytes
Antigens
Polymerase Chain Reaction

Keywords

  • aging
  • BCL2
  • non-Hodgkin lymphoma
  • PCR
  • somatic mutation

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

BCL2 translocation frequency rises with age in humans. / Liu, Yafei; Hernandez, Antonio M.; Shibata, Darryl; Cortopassi, Gino A.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, No. 19, 13.09.1994, p. 8910-8914.

Research output: Contribution to journalArticle

Liu, Yafei ; Hernandez, Antonio M. ; Shibata, Darryl ; Cortopassi, Gino A. / BCL2 translocation frequency rises with age in humans. In: Proceedings of the National Academy of Sciences of the United States of America. 1994 ; Vol. 91, No. 19. pp. 8910-8914.
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