Basic fibroblast growth factor prevents cAMP-induced apoptosis in cultured schwann cells

R. Shaw, R. Cianchetti, David E Pleasure, B. Kreider

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Abstract

Peripheral nerve axotomy induces apoptosis in Schwann cell precursors; basic fibroblast growth factor (bFGF) protects these cells from axotomy- induced apoptosis (Jessen et al.: Neuron 12:509-527, 1994; Gavrilovic et al.: Eur J Neurosci 7:7-85, 1995). In this study, we investigate the effects of bFGF on apoptosis in neuron-free cultures of neonatal rat Schwann cells. Apoptotic cell death was induced in primary and secondary expanded Schwann cells by treatment with 1 mM concentrations of 8-bromoadenosine 3':5'- cyclic monophosphate (8-bromo-cAMP), a membrane-permeable analogue of cAMP which induces expression of galactocerebroside in the plasma membranes of Schwann cells. Treatment with bFGF reduced the percentage of galactocerebroside-bearing Schwann cells undergoing cAMP-induced DNA fragmentation. These findings suggest that bFGF can enhance the survival of terminally differentiated Schwann cells by preventing apoptosis.

Original languageEnglish (US)
Pages (from-to)400-404
Number of pages5
JournalJournal of Neuroscience Research
Volume47
Issue number4
DOIs
StatePublished - Feb 15 1997
Externally publishedYes

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Keywords

  • apoptosis
  • bFGF
  • cAMP
  • Schwann cell

ASJC Scopus subject areas

  • Neuroscience(all)

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