Peripheral nerve axotomy induces apoptosis in Schwann cell precursors; basic fibroblast growth factor (bFGF) protects these cells from axotomy- induced apoptosis (Jessen et al.: Neuron 12:509-527, 1994; Gavrilovic et al.: Eur J Neurosci 7:7-85, 1995). In this study, we investigate the effects of bFGF on apoptosis in neuron-free cultures of neonatal rat Schwann cells. Apoptotic cell death was induced in primary and secondary expanded Schwann cells by treatment with 1 mM concentrations of 8-bromoadenosine 3':5'- cyclic monophosphate (8-bromo-cAMP), a membrane-permeable analogue of cAMP which induces expression of galactocerebroside in the plasma membranes of Schwann cells. Treatment with bFGF reduced the percentage of galactocerebroside-bearing Schwann cells undergoing cAMP-induced DNA fragmentation. These findings suggest that bFGF can enhance the survival of terminally differentiated Schwann cells by preventing apoptosis.
|Original language||English (US)|
|Number of pages||5|
|Journal||Journal of Neuroscience Research|
|State||Published - Feb 15 1997|
- Schwann cell
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