Basic fibroblast growth factor improves trabecular bone connectivity and bone strength in the lumbar vertebral body of osteopenic rats

Wei Yao, Tamer Hadi, Yebin Jiang, Jeff Lotz, Thomas J. Wronski, Nancy E Lane

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Recently, basic fibroblast growth factor (bFGF) has been found to increase trabecular bone mass and connectivity in the proximal tibial metaphyses (PTM) in osteopenic rats. The purpose of this study was to determine the bone anabolic effects of bFGF in the lumbar vertebral body (LVB), a less loaded skeletal site with a lower rate of bone turnover than the PTM. Six-month old female Sprague-Dawley rats were ovariectomized (OVX) or sham-operated and untreated for 8 weeks to induce osteopenia. Then group 1 (sham) and group 2 (OVX) were treated subcutaneously (SC) with vehicle, and OVXed groups 3 and 4 were treated SC with PTH [hPTH (1-34) at 40 μg/kg, 5x/week] and bFGF (1 mg/kg, 5x/week), respectively, for 8 weeks. At sacrifice, the fifth LVB was removed, subjected to micro-CT for determination of trabecular bone structure and then processed for histomorphometry to assess bone turnover. The sixth LVB was used for mechanical compression testing (MTS, Bionix 858). The data were analyzed with the Kruskal-Wallis test followed by post-hoc testing as needed. After 16 weeks of estrogen deficiency, there were significant reductions in vertebral trabecular bone volume and trabecular thickness. Treatment with either bFGF or hPTH (1-34) increased BV/TV in OVX animals. Human PTH (1-34)-treated animals had significant increases in trabecular (48%) and cortical thickness (30%) and bone strength [maximum load (53%) and work to failure (175%)] compared to OVX + Vehicle animals. Treatment of osteopenic rats with bFGF increased bone volume (15%), trabecular thickness (13%), maximum load (45%) and work to failure (140%) compared to OVX + Vehicle animals (all P < 0.05). Basic FGF increased trabecular bone volume in the lumbar vertebral body of osteopenic rats by restoring trabecular number, thickness and connectivity density. Also, bFGF improved bone mechanical properties (maximum force and work to failure) compared to the OVX + Vehicle group. Therefore, increasing the number, thickness and connections of the trabeculae contributes to increased bone strength in this small animal model of osteoporosis.

Original languageEnglish (US)
Pages (from-to)1939-1947
Number of pages9
JournalOsteoporosis International
Volume16
Issue number12
DOIs
StatePublished - Dec 2005

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Fibroblast Growth Factor 2
Bone and Bones
Teriparatide
Bone Remodeling
Workload
Parathyroid Hormone
Fibroblast Growth Factor 1
Anabolic Agents
Metabolic Bone Diseases
Osteoporosis
Sprague Dawley Rats
Cancellous Bone
Estrogens
Animal Models

Keywords

  • bFGF
  • Biomechanical properties
  • Bone structure
  • Lumber vertebrae
  • Ovariectomy
  • Rat

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Basic fibroblast growth factor improves trabecular bone connectivity and bone strength in the lumbar vertebral body of osteopenic rats. / Yao, Wei; Hadi, Tamer; Jiang, Yebin; Lotz, Jeff; Wronski, Thomas J.; Lane, Nancy E.

In: Osteoporosis International, Vol. 16, No. 12, 12.2005, p. 1939-1947.

Research output: Contribution to journalArticle

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