Basal body stability and ciliogenesis requires the conserved component Poc1

Chad G. Pearson, Daniel P S Osborn, Thomas H. Giddings, Philip L. Beales, Mark Winey

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Centrioles are the foundation for centrosome and cilia formation. The biogenesis of centrioles is initiated by an assembly mechanism that first synthesizes the ninefold symmetrical cartwheel and subsequently leads to a stable cylindrical microtubule scaffold that is capable of withstanding microtubule-based forces generated by centrosomes and cilia. We report that the conserved WD40 repeat domain-containing cartwheel protein Poc1 is required for the structural maintenance of centrioles in Tetrahymena thermophila. Furthermore, human Poc1B is required for primary ciliogenesis, and in zebrafish, DrPoc1B knockdown causes ciliary defects and morphological phenotypes consistent with human ciliopathies. T. thermophila Poc1 exhibits a protein incorporation profile commonly associated with structural centriole components in which the majority of Poc1 is stably incorporated during new centriole assembly. A second dynamic population assembles throughout the cell cycle. Our experiments identify novel roles for Poc1 in centriole stability and ciliogenesis.

Original languageEnglish (US)
Pages (from-to)905-920
Number of pages16
JournalJournal of Cell Biology
Volume187
Issue number6
DOIs
StatePublished - Dec 14 2009
Externally publishedYes

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Basal Bodies
Centrioles
Tetrahymena thermophila
Centrosome
Cilia
Microtubules
Population Dynamics
Zebrafish
Cell Cycle
Proteins
Maintenance
Phenotype

ASJC Scopus subject areas

  • Cell Biology
  • Medicine(all)

Cite this

Basal body stability and ciliogenesis requires the conserved component Poc1. / Pearson, Chad G.; Osborn, Daniel P S; Giddings, Thomas H.; Beales, Philip L.; Winey, Mark.

In: Journal of Cell Biology, Vol. 187, No. 6, 14.12.2009, p. 905-920.

Research output: Contribution to journalArticle

Pearson, Chad G. ; Osborn, Daniel P S ; Giddings, Thomas H. ; Beales, Philip L. ; Winey, Mark. / Basal body stability and ciliogenesis requires the conserved component Poc1. In: Journal of Cell Biology. 2009 ; Vol. 187, No. 6. pp. 905-920.
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