Basal body duplication and maintenance require one member of the Tetrahymena thermophila centrin gene family

Alexander J. Stemm-Wolf, Garry Morgan, Thomas H. Giddings, Erin A. White, Robb Marchione, Heather B. McDonald, Mark Winey

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Centrins, small calcium binding EF-hand proteins, function in the duplication of a variety of microtubule organizing centers. These include centrioles in humans, basal bodies in green algae, and spindle pole bodies in yeast. The ciliate Tetrahymena thermophila contains at least four centrin genes as determined by sequence homology, and these have distinct localization and expression patterns. CEN1's role at the basal body was examined more closely. The Cen1 protein localizes primarily to two locations: one is the site at the base of the basal body where duplication is initiated. The other is the transition zone between the basal body and axoneme. CEN1 is an essential gene, the deletion of which results in the loss of basal bodies, which is likely due to defects in both basal body duplication and basal body maintenance. Analysis of the three other centrins indicates that two of them function at microtubule-rich structures unique to ciliates, whereas the fourth is not expressed under conditions examined in this study, although when artificially expressed it localizes to basal bodies. This study provides evidence that in addition to its previously known function in the duplication of basal bodies, centrin is also important for the integrity of these organelles.

Original languageEnglish (US)
Pages (from-to)3606-3619
Number of pages14
JournalMolecular Biology of the Cell
Volume16
Issue number8
DOIs
StatePublished - Aug 1 2005
Externally publishedYes

Fingerprint

Tetrahymena thermophila
Basal Bodies
Sulfamethoxazole Drug Combination Trimethoprim
Maintenance
Genes
Spindle Pole Bodies
Microtubule-Organizing Center
Axoneme
Centrioles
EF Hand Motifs
Chlorophyta
Essential Genes
Gene Deletion
Sequence Homology
Human Body
Microtubules
Organelles
Proteins
Yeasts
Calcium

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Basal body duplication and maintenance require one member of the Tetrahymena thermophila centrin gene family. / Stemm-Wolf, Alexander J.; Morgan, Garry; Giddings, Thomas H.; White, Erin A.; Marchione, Robb; McDonald, Heather B.; Winey, Mark.

In: Molecular Biology of the Cell, Vol. 16, No. 8, 01.08.2005, p. 3606-3619.

Research output: Contribution to journalArticle

Stemm-Wolf, Alexander J. ; Morgan, Garry ; Giddings, Thomas H. ; White, Erin A. ; Marchione, Robb ; McDonald, Heather B. ; Winey, Mark. / Basal body duplication and maintenance require one member of the Tetrahymena thermophila centrin gene family. In: Molecular Biology of the Cell. 2005 ; Vol. 16, No. 8. pp. 3606-3619.
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