Bartonella alsatica sp. nov., a new Bartonella species isolated from the blood of wild rabbits

Rémy Heller, Maryline Kubina, Philippe Mariet, Philippe Riegel, Gilles Delacour, Christoph Dehio, François Lamarque, Rick Kasten, Henri Jean Boulouis, Henri Monteil, Bruno B Chomel, Yves Piémont

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Bartonella species are considered as emerging human pathogens, with at least six different species pathogenic or possibly pathogenic for humans. However, little is known about Bartonella distribution, species polymorphism and pathogenicity in mammalian species. The objective of this work was to determine the presence, the frequency and the distribution of Bartonella species in wild rabbits (Oryctolagus cuniculus) caught in warrens in Alsace, France. Humans may come into contact with wild rabbits when hunting, especially when they are picked up with bare hands and at time of evisceration. Of 30 blood samples collected and cultured from wild rabbits, nine (30%) were positive for organisms morphologically similar to Bartonella spp. The bacteria appeared as small, fastidious, aerobic, oxidase-negative, Gram-negative rods which could be localized within erythrocytes. Their biochemical properties were similar to those of the genus Bartonella. The sequence of the 16S rRNA gene obtained from the rabbit isolates was highly related to the sequences of the different Bartonella species (97.8-99.3 % similarity). The high DNA hybridization rate (81-90% similarity) between the three strains isolated from rabbit blood confirmed that they belong to the same bacterial species. Hybridization values, obtained with the nuclease-TCA method, when testing type strains of recognized Bartonella species (9-14% similarity), support the creation of a new species for the rabbit isolates. The name Bartonella alsatica is proposed for these strains isolated from the blood of wild rabbits. The type strain is lBS 382(T) (= CIP 105477(T)).

Original languageEnglish (US)
Pages (from-to)283-288
Number of pages6
JournalInternational Journal of Systematic Bacteriology
Volume49
Issue number1
StatePublished - Jan 1999
Externally publishedYes

Fingerprint

Bartonella alsatica
Bartonella
blood
rabbits
new species
Rabbits
France
evisceration
Oryctolagus cuniculus
nucleases
testing method
nucleic acid hybridization
pathogenicity
rRNA Genes
hunting
hands
erythrocytes
hybridization
Names
biogeography

Keywords

  • Bartonella
  • Bartonella alsatica
  • Oryctolagus cuniculus
  • Wild rabbit
  • Zoonoses

ASJC Scopus subject areas

  • Microbiology
  • Immunology

Cite this

Heller, R., Kubina, M., Mariet, P., Riegel, P., Delacour, G., Dehio, C., ... Piémont, Y. (1999). Bartonella alsatica sp. nov., a new Bartonella species isolated from the blood of wild rabbits. International Journal of Systematic Bacteriology, 49(1), 283-288.

Bartonella alsatica sp. nov., a new Bartonella species isolated from the blood of wild rabbits. / Heller, Rémy; Kubina, Maryline; Mariet, Philippe; Riegel, Philippe; Delacour, Gilles; Dehio, Christoph; Lamarque, François; Kasten, Rick; Boulouis, Henri Jean; Monteil, Henri; Chomel, Bruno B; Piémont, Yves.

In: International Journal of Systematic Bacteriology, Vol. 49, No. 1, 01.1999, p. 283-288.

Research output: Contribution to journalArticle

Heller, R, Kubina, M, Mariet, P, Riegel, P, Delacour, G, Dehio, C, Lamarque, F, Kasten, R, Boulouis, HJ, Monteil, H, Chomel, BB & Piémont, Y 1999, 'Bartonella alsatica sp. nov., a new Bartonella species isolated from the blood of wild rabbits', International Journal of Systematic Bacteriology, vol. 49, no. 1, pp. 283-288.
Heller, Rémy ; Kubina, Maryline ; Mariet, Philippe ; Riegel, Philippe ; Delacour, Gilles ; Dehio, Christoph ; Lamarque, François ; Kasten, Rick ; Boulouis, Henri Jean ; Monteil, Henri ; Chomel, Bruno B ; Piémont, Yves. / Bartonella alsatica sp. nov., a new Bartonella species isolated from the blood of wild rabbits. In: International Journal of Systematic Bacteriology. 1999 ; Vol. 49, No. 1. pp. 283-288.
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