Banking placental tissue: An optimized collection procedure for genome-wide analysis of nucleic acids

L. M. Wolfe, R. D. Thiagarajan, F. Boscolo, Veronique Tache, R. L. Coleman, J. Kim, W. K. Kwan, J. F. Loring, M. Parast, L. C. Laurent

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Introduction Banking of high-quality placental tissue specimens will enable biomarker discovery and molecular studies on diseases involving placental dysfunction. Systematic studies aimed at developing feasible standardized methodology for placental collection in a typical clinical setting are lacking. Methods To determine the acceptable timeframe for placental collection, we collected multiple samples from first and third trimester placentas at serial timepoints in a 2-h window after delivery, simultaneously comparing the traditional snap-freeze technique to commercial solutions designed to preserve RNA (RNAlater™), and DNA (DNAgard®). The performance of RNAlater for preserving DNA was also tested. Nucleic acid quality was assessed by determining the RNA integrity number (RIN) and genome-wide microarray profiling for gene expression and DNA methylation. Results We found that samples collected in RNAlater had higher and more consistent RINs compared to snap-frozen tissue. Similar RINs were obtained for tissue collected in RNAlater as large (1 cm3) and small (∼0.1 cm3) pieces. RNAlater appeared to better stabilize the time zero gene expression profile compared to snap-freezing for first trimester placenta. DNA methylation profiles remained quite stable over a 2 h time period after removal of the placenta from the uterus, with DNAgard being superior to other treatments. Discussion and conclusion The collection of placental samples in RNAlater and DNAgard is simple, and eliminates the need for liquid nitrogen or a freezer on-site. Moreover, the quality of the nucleic acids and the resulting data from samples collected in these preservation solutions is higher than samples collected using the snap-freeze method and easier to implement in busy clinical environments.

Original languageEnglish (US)
Pages (from-to)645-654
Number of pages10
JournalPlacenta
Volume35
Issue number8
DOIs
StatePublished - 2014

Fingerprint

Tissue Banks
Nucleic Acids
Placenta
Genome
DNA Methylation
First Pregnancy Trimester
Placenta Diseases
RNA
DNA
Third Pregnancy Trimester
Gene Expression Profiling
Transcriptome
Freezing
Uterus
Nitrogen
Biomarkers

Keywords

  • DNA methylation
  • DNAgard
  • Gene expression
  • Microarray
  • Placenta
  • RNA integrity
  • RNAlater
  • Snap-freezing

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Reproductive Medicine
  • Developmental Biology

Cite this

Wolfe, L. M., Thiagarajan, R. D., Boscolo, F., Tache, V., Coleman, R. L., Kim, J., ... Laurent, L. C. (2014). Banking placental tissue: An optimized collection procedure for genome-wide analysis of nucleic acids. Placenta, 35(8), 645-654. https://doi.org/10.1016/j.placenta.2014.05.005

Banking placental tissue : An optimized collection procedure for genome-wide analysis of nucleic acids. / Wolfe, L. M.; Thiagarajan, R. D.; Boscolo, F.; Tache, Veronique; Coleman, R. L.; Kim, J.; Kwan, W. K.; Loring, J. F.; Parast, M.; Laurent, L. C.

In: Placenta, Vol. 35, No. 8, 2014, p. 645-654.

Research output: Contribution to journalArticle

Wolfe, LM, Thiagarajan, RD, Boscolo, F, Tache, V, Coleman, RL, Kim, J, Kwan, WK, Loring, JF, Parast, M & Laurent, LC 2014, 'Banking placental tissue: An optimized collection procedure for genome-wide analysis of nucleic acids', Placenta, vol. 35, no. 8, pp. 645-654. https://doi.org/10.1016/j.placenta.2014.05.005
Wolfe, L. M. ; Thiagarajan, R. D. ; Boscolo, F. ; Tache, Veronique ; Coleman, R. L. ; Kim, J. ; Kwan, W. K. ; Loring, J. F. ; Parast, M. ; Laurent, L. C. / Banking placental tissue : An optimized collection procedure for genome-wide analysis of nucleic acids. In: Placenta. 2014 ; Vol. 35, No. 8. pp. 645-654.
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AU - Tache, Veronique

AU - Coleman, R. L.

AU - Kim, J.

AU - Kwan, W. K.

AU - Loring, J. F.

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AU - Laurent, L. C.

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