Balloon catheterization induces arterial expression of embryonic fibronectins

D. Dubin, J. H. Peters, L. F. Brown, B. Logan, K. C. Kent, B. Berse, S. Berven, B. Cercek, B. G. Sharifi, R. E. Pratt, V. J. Dzau, L. Van De Water

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Fibronectins (FNs) comprise a family of adhesive extracellular matrix proteins that arise by alternative splicing in three regions: V (IIICS), EIIIA (ED-A), and EIIIB (ED-B). FNs bearing the EIIIA and EIIIB segments are prevalent during embryogenesis, expressed to lesser degrees in normal adult tissues, and may be locally reexpressed at sites of adult tissue injury. RNase mapping shows that normal rat arteries express low levels of FNs that are predominantly EIIIA and EIIIB. Following balloon injury, arterial walls produce increased total levels of FN transcripts that preferentially include both the EIIIA and EIIIB segments. However, despite inducing increased total FN mRNA, balloon injury does not alter the relative composition of V120+, V95+, and V0 spliced forms. In situ hybridization reveals that as early as 4 days after injury medial cells express increased total FN mRNA, and by 7 days substantial neointimal and focal medial synthesis of EIIIA+, EIIIB+, and V120+ FNs occurs; macrophages do not significantly contribute to this observed vascular FN synthesis. Consistent with the mRNA data, immunofluorescence microscopic analysis reveals increased deposition of EIIIB+ and V+ FN protein forms in injured arterial walls, particularly within the neointima. Our results suggest that local synthesis of specific FN isoforms is important to the neointimal formation that ensues after balloon injury.

Original languageEnglish (US)
Pages (from-to)1958-1967
Number of pages10
JournalArteriosclerosis, Thrombosis, and Vascular Biology
Volume15
Issue number11
StatePublished - 1995
Externally publishedYes

Fingerprint

Fibronectins
Catheterization
Wounds and Injuries
Messenger RNA
Neointima
Extracellular Matrix Proteins
Alternative Splicing
Ribonucleases
Adhesives
Embryonic Development
In Situ Hybridization
Fluorescent Antibody Technique
Blood Vessels
Protein Isoforms
Arteries
Macrophages

Keywords

  • alternative splicing
  • artery
  • fibronectins
  • macrophages
  • neointima

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Dubin, D., Peters, J. H., Brown, L. F., Logan, B., Kent, K. C., Berse, B., ... Van De Water, L. (1995). Balloon catheterization induces arterial expression of embryonic fibronectins. Arteriosclerosis, Thrombosis, and Vascular Biology, 15(11), 1958-1967.

Balloon catheterization induces arterial expression of embryonic fibronectins. / Dubin, D.; Peters, J. H.; Brown, L. F.; Logan, B.; Kent, K. C.; Berse, B.; Berven, S.; Cercek, B.; Sharifi, B. G.; Pratt, R. E.; Dzau, V. J.; Van De Water, L.

In: Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 15, No. 11, 1995, p. 1958-1967.

Research output: Contribution to journalArticle

Dubin, D, Peters, JH, Brown, LF, Logan, B, Kent, KC, Berse, B, Berven, S, Cercek, B, Sharifi, BG, Pratt, RE, Dzau, VJ & Van De Water, L 1995, 'Balloon catheterization induces arterial expression of embryonic fibronectins', Arteriosclerosis, Thrombosis, and Vascular Biology, vol. 15, no. 11, pp. 1958-1967.
Dubin D, Peters JH, Brown LF, Logan B, Kent KC, Berse B et al. Balloon catheterization induces arterial expression of embryonic fibronectins. Arteriosclerosis, Thrombosis, and Vascular Biology. 1995;15(11):1958-1967.
Dubin, D. ; Peters, J. H. ; Brown, L. F. ; Logan, B. ; Kent, K. C. ; Berse, B. ; Berven, S. ; Cercek, B. ; Sharifi, B. G. ; Pratt, R. E. ; Dzau, V. J. ; Van De Water, L. / Balloon catheterization induces arterial expression of embryonic fibronectins. In: Arteriosclerosis, Thrombosis, and Vascular Biology. 1995 ; Vol. 15, No. 11. pp. 1958-1967.
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