BAFF/BAFF-R involved in antibodies production of rats with collagen-induced arthritis via PI3K-Akt-mTOR signaling and the regulation of paeoniflorin

Pei Pei Li, Dan Dan Liu, Yun Jie Liu, Shan Shan Song, Qingtong Wang, Yan Chang, Yu Jing Wu, Jing Yu Chen, Wen Di Zhao, Ling Ling Zhang, Wei Wei

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39 Scopus citations

Abstract

Ethnopharmacological relevance: Paeoniflorin (Pae) is extracted from the root of paeonia lactiflora which have attracted attention for anti-rheumatic and immune modulating properties. Aim of the study: To investigate the role of PI3K/Akt/mTOR signaling mediated by BAFF/BAFF-R in antibodies production and the regulation of Pae on the signaling pathway in rats with collagen-induced arthritis (CIA). Materials and methods: CIA rats were randomly separated into different groups and treated with Pae (25, 100 mg/kg) from day 18 to day 38 after immunization. The effects of Pae on B lymphocytes of CIA rats were evaluated by the levels of BAFF, anti-CII antibody, IgA, IgG and IgM, and the expressions of BAFF-R, PI3K, p-Akt and mTOR. Results: In CIA rats, the levels of anti-CII antibody, IgA, IgG and IgM in serum enhanced, BAFF, BAFF-R, PI3K, p-Akt and mTOR were highly expressed. Pae (100 mg/kg) obviously decreased arthritis score, relieved ankle and paw swelling, improved spleen histopathology in CIA rats, decreased the levels of IgA, IgM, IgG and anti-CII antibody, and significantly decreased the expressions of BAFF, BAFF-R, PI3K, p-Akt and mTOR. Conclusion: PI3K/Akt/mTOR signaling mediated by BAFF/BAFF-R participates in antibodies production by B lymphocytes of CIA rats. Pae had therapeutic effects on rats with CIA. These effects might be relative to regulating PI3K/Akt/mTOR signal mediated by BAFF/BAFF-R, and down regulate the antibodies production further.

Original languageEnglish (US)
Pages (from-to)290-300
Number of pages11
JournalJournal of Ethnopharmacology
Volume141
Issue number1
DOIs
StatePublished - May 7 2012
Externally publishedYes

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Keywords

  • BAFF/BAFF-R
  • Collagen induced arthritis
  • Paeoniflorin
  • PI3K/Akt/mTOR
  • Signaling

ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery

Cite this

Li, P. P., Liu, D. D., Liu, Y. J., Song, S. S., Wang, Q., Chang, Y., Wu, Y. J., Chen, J. Y., Zhao, W. D., Zhang, L. L., & Wei, W. (2012). BAFF/BAFF-R involved in antibodies production of rats with collagen-induced arthritis via PI3K-Akt-mTOR signaling and the regulation of paeoniflorin. Journal of Ethnopharmacology, 141(1), 290-300. https://doi.org/10.1016/j.jep.2012.02.034