BAFF upregulates CD28/B7 and CD40/CD154 expression and promotes mouse T and B cell interaction in vitro via BAFF receptor

Feng Zhang, Shan Shan Song, Jin Ling Shu, Ying Li, Yu Jing Wu, Qingtong Wang, Jing Yu Chen, Yan Chang, Hua Xun Wu, Ling Ling Zhang, Wei Wei

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Aim:B cell-activating factor belonging to the TNF family (BAFF) is a member of TNF family and required for peripheral B cell survival and homeostasis. BAFF has been shown to promote the proliferation of T and B cells. In this study we examined whether and how BAFF mediated the interaction between mouse T and B cells in vitro.Methods:BAFF-stimulated B or T cells were co-cultured with T or B cells. The interactions between T and B cells were analyzed by measuring the expression of co-stimulatory molecules (CD28/CD80 or CD40/CD154), the proliferation and secretion of T and B cells and other factors. Two siRNAs against the transmembrane activator and calcium modulator and cyclophilin ligand interactor (TACI) and BAFF receptor (BAFF-R) were used to identify the receptors responsible for the actions of BAFF.Results:BAFF-stimulated B cells significantly promoted the proliferation and activity of co-cultured T cells, and increased the percentages of CD4+ CD28+ and CD4+ CD154+ T cells. Similarly, BAFF-stimulated T cells significantly promoted the proliferation and activity of co-cultured B cells, and increased CD19+ CD80+ and CD19+ CD40+ B cell subpopulations. BAFF-R siRNA-silenced B cells showed significantly lower expression of CD40 and CD80 than the control B cells. When the BAFF-R siRNA-silenced B cells were stimulated with BAFF, then co-cultured with T cells, the expression of CD28 and CD154 on T cells was not increased. TACI siRNA-silenced B cells exhibited higher expression of CD40 and CD80 than the control B cells. When the TACI siRNA-silenced B cells were stimulated with BAFF, then co-cultured with T cells, the expression of CD28 and CD154 on T cells was significantly increased.Conclusion:BAFF upregulates CD28/B7 and CD40/CD154 expression, and promotes the interactions between T and B cells in a BAFF-R-dependent manner.

Original languageEnglish (US)
Pages (from-to)1101-1109
Number of pages9
JournalActa Pharmacologica Sinica
Volume37
Issue number8
DOIs
StatePublished - Aug 1 2016
Externally publishedYes

Fingerprint

B-Cell Activation Factor Receptor
Cell Communication
B-Lymphocytes
Up-Regulation
T-Lymphocytes
Small Interfering RNA
In Vitro Techniques
Transmembrane Activator and CAML Interactor Protein
Cultured Cells
B-Cell Activating Factor

Keywords

  • B cells
  • BAFF
  • BAFF receptors
  • co-stimulatory molecules
  • T and B cell interaction
  • T cells
  • TACI

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)

Cite this

BAFF upregulates CD28/B7 and CD40/CD154 expression and promotes mouse T and B cell interaction in vitro via BAFF receptor. / Zhang, Feng; Song, Shan Shan; Shu, Jin Ling; Li, Ying; Wu, Yu Jing; Wang, Qingtong; Chen, Jing Yu; Chang, Yan; Wu, Hua Xun; Zhang, Ling Ling; Wei, Wei.

In: Acta Pharmacologica Sinica, Vol. 37, No. 8, 01.08.2016, p. 1101-1109.

Research output: Contribution to journalArticle

Zhang, F, Song, SS, Shu, JL, Li, Y, Wu, YJ, Wang, Q, Chen, JY, Chang, Y, Wu, HX, Zhang, LL & Wei, W 2016, 'BAFF upregulates CD28/B7 and CD40/CD154 expression and promotes mouse T and B cell interaction in vitro via BAFF receptor', Acta Pharmacologica Sinica, vol. 37, no. 8, pp. 1101-1109. https://doi.org/10.1038/aps.2016.15
Zhang, Feng ; Song, Shan Shan ; Shu, Jin Ling ; Li, Ying ; Wu, Yu Jing ; Wang, Qingtong ; Chen, Jing Yu ; Chang, Yan ; Wu, Hua Xun ; Zhang, Ling Ling ; Wei, Wei. / BAFF upregulates CD28/B7 and CD40/CD154 expression and promotes mouse T and B cell interaction in vitro via BAFF receptor. In: Acta Pharmacologica Sinica. 2016 ; Vol. 37, No. 8. pp. 1101-1109.
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abstract = "Aim:B cell-activating factor belonging to the TNF family (BAFF) is a member of TNF family and required for peripheral B cell survival and homeostasis. BAFF has been shown to promote the proliferation of T and B cells. In this study we examined whether and how BAFF mediated the interaction between mouse T and B cells in vitro.Methods:BAFF-stimulated B or T cells were co-cultured with T or B cells. The interactions between T and B cells were analyzed by measuring the expression of co-stimulatory molecules (CD28/CD80 or CD40/CD154), the proliferation and secretion of T and B cells and other factors. Two siRNAs against the transmembrane activator and calcium modulator and cyclophilin ligand interactor (TACI) and BAFF receptor (BAFF-R) were used to identify the receptors responsible for the actions of BAFF.Results:BAFF-stimulated B cells significantly promoted the proliferation and activity of co-cultured T cells, and increased the percentages of CD4+ CD28+ and CD4+ CD154+ T cells. Similarly, BAFF-stimulated T cells significantly promoted the proliferation and activity of co-cultured B cells, and increased CD19+ CD80+ and CD19+ CD40+ B cell subpopulations. BAFF-R siRNA-silenced B cells showed significantly lower expression of CD40 and CD80 than the control B cells. When the BAFF-R siRNA-silenced B cells were stimulated with BAFF, then co-cultured with T cells, the expression of CD28 and CD154 on T cells was not increased. TACI siRNA-silenced B cells exhibited higher expression of CD40 and CD80 than the control B cells. When the TACI siRNA-silenced B cells were stimulated with BAFF, then co-cultured with T cells, the expression of CD28 and CD154 on T cells was significantly increased.Conclusion:BAFF upregulates CD28/B7 and CD40/CD154 expression, and promotes the interactions between T and B cells in a BAFF-R-dependent manner.",
keywords = "B cells, BAFF, BAFF receptors, co-stimulatory molecules, T and B cell interaction, T cells, TACI",
author = "Feng Zhang and Song, {Shan Shan} and Shu, {Jin Ling} and Ying Li and Wu, {Yu Jing} and Qingtong Wang and Chen, {Jing Yu} and Yan Chang and Wu, {Hua Xun} and Zhang, {Ling Ling} and Wei Wei",
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TY - JOUR

T1 - BAFF upregulates CD28/B7 and CD40/CD154 expression and promotes mouse T and B cell interaction in vitro via BAFF receptor

AU - Zhang, Feng

AU - Song, Shan Shan

AU - Shu, Jin Ling

AU - Li, Ying

AU - Wu, Yu Jing

AU - Wang, Qingtong

AU - Chen, Jing Yu

AU - Chang, Yan

AU - Wu, Hua Xun

AU - Zhang, Ling Ling

AU - Wei, Wei

PY - 2016/8/1

Y1 - 2016/8/1

N2 - Aim:B cell-activating factor belonging to the TNF family (BAFF) is a member of TNF family and required for peripheral B cell survival and homeostasis. BAFF has been shown to promote the proliferation of T and B cells. In this study we examined whether and how BAFF mediated the interaction between mouse T and B cells in vitro.Methods:BAFF-stimulated B or T cells were co-cultured with T or B cells. The interactions between T and B cells were analyzed by measuring the expression of co-stimulatory molecules (CD28/CD80 or CD40/CD154), the proliferation and secretion of T and B cells and other factors. Two siRNAs against the transmembrane activator and calcium modulator and cyclophilin ligand interactor (TACI) and BAFF receptor (BAFF-R) were used to identify the receptors responsible for the actions of BAFF.Results:BAFF-stimulated B cells significantly promoted the proliferation and activity of co-cultured T cells, and increased the percentages of CD4+ CD28+ and CD4+ CD154+ T cells. Similarly, BAFF-stimulated T cells significantly promoted the proliferation and activity of co-cultured B cells, and increased CD19+ CD80+ and CD19+ CD40+ B cell subpopulations. BAFF-R siRNA-silenced B cells showed significantly lower expression of CD40 and CD80 than the control B cells. When the BAFF-R siRNA-silenced B cells were stimulated with BAFF, then co-cultured with T cells, the expression of CD28 and CD154 on T cells was not increased. TACI siRNA-silenced B cells exhibited higher expression of CD40 and CD80 than the control B cells. When the TACI siRNA-silenced B cells were stimulated with BAFF, then co-cultured with T cells, the expression of CD28 and CD154 on T cells was significantly increased.Conclusion:BAFF upregulates CD28/B7 and CD40/CD154 expression, and promotes the interactions between T and B cells in a BAFF-R-dependent manner.

AB - Aim:B cell-activating factor belonging to the TNF family (BAFF) is a member of TNF family and required for peripheral B cell survival and homeostasis. BAFF has been shown to promote the proliferation of T and B cells. In this study we examined whether and how BAFF mediated the interaction between mouse T and B cells in vitro.Methods:BAFF-stimulated B or T cells were co-cultured with T or B cells. The interactions between T and B cells were analyzed by measuring the expression of co-stimulatory molecules (CD28/CD80 or CD40/CD154), the proliferation and secretion of T and B cells and other factors. Two siRNAs against the transmembrane activator and calcium modulator and cyclophilin ligand interactor (TACI) and BAFF receptor (BAFF-R) were used to identify the receptors responsible for the actions of BAFF.Results:BAFF-stimulated B cells significantly promoted the proliferation and activity of co-cultured T cells, and increased the percentages of CD4+ CD28+ and CD4+ CD154+ T cells. Similarly, BAFF-stimulated T cells significantly promoted the proliferation and activity of co-cultured B cells, and increased CD19+ CD80+ and CD19+ CD40+ B cell subpopulations. BAFF-R siRNA-silenced B cells showed significantly lower expression of CD40 and CD80 than the control B cells. When the BAFF-R siRNA-silenced B cells were stimulated with BAFF, then co-cultured with T cells, the expression of CD28 and CD154 on T cells was not increased. TACI siRNA-silenced B cells exhibited higher expression of CD40 and CD80 than the control B cells. When the TACI siRNA-silenced B cells were stimulated with BAFF, then co-cultured with T cells, the expression of CD28 and CD154 on T cells was significantly increased.Conclusion:BAFF upregulates CD28/B7 and CD40/CD154 expression, and promotes the interactions between T and B cells in a BAFF-R-dependent manner.

KW - B cells

KW - BAFF

KW - BAFF receptors

KW - co-stimulatory molecules

KW - T and B cell interaction

KW - T cells

KW - TACI

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