BAFF promotes T cell activation through the BAFF-BAFF-R-PI3K-Akt signaling pathway

Shanshan Hu, Rui Wang, Mei Zhang, Kangkang Liu, Juan Tao, Yu Tai, Weijie Zhou, Qingtong Wang, Wei Wei

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

B-cell activating factor from the tumor necrosis factor family (BAFF) has revealed its critical role in B cell proliferation and survival, as well as the pathogenesis of T-cell mediated autoimmune disease. However, the effect and molecular mechanisms of BAFF on T cell physiological function have not been fully elucidated. In this study it was seen that BAFF can promote the vitality of purified T cells, increase the proportion of CD3 + CD4 + , CD4 + CD25 + , CD4 + CD154 + , and CD4 + CD69 + subgroups and reduce the proportion of CD4 + CD62L + subgroups. Negating BAFF activity with Atacicept (TACI-Fc) reverses vitality and activation of T cells. Furthermore, immunofluorescence detection revealed that BAFF promotes the expression of BAFF receptor (BAFF-R) and transmembrane activator and CAML interactor (TACI) in T cells. Flow cytometry displayed that BAFF/BAFF-R activates the PI3K-Akt signaling pathway while the application of PI3K inhibitor (wortmannin) illuminated that BAFF induces T cell vitality and activation through the PI3K-Akt signaling pathway. We conclude that BAFF is involved in not only the physiology of B cells, but also that of T cells. BAFF affects physiological T-cell activation through BAFF-R-mediated activation of the PI3K-Akt signaling pathway which mirrors one of the pathological mechanisms of T cell-mediated autoimmune diseases.

Original languageEnglish (US)
Article number108796
JournalBiomedicine and Pharmacotherapy
Volume114
DOIs
StatePublished - Jun 1 2019
Externally publishedYes

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Keywords

  • B-cell activating factor from the tumor necrosis factor family (BAFF)
  • B-cell activating factor from the tumor necrosis factor family receptor (BAFF-R)
  • Phosphoinositide 3-kinase (PI3K)
  • T cell activation
  • Transmembrane activator and CAML interactor (TACI)

ASJC Scopus subject areas

  • Pharmacology

Cite this

Hu, S., Wang, R., Zhang, M., Liu, K., Tao, J., Tai, Y., Zhou, W., Wang, Q., & Wei, W. (2019). BAFF promotes T cell activation through the BAFF-BAFF-R-PI3K-Akt signaling pathway. Biomedicine and Pharmacotherapy, 114, [108796]. https://doi.org/10.1016/j.biopha.2019.108796