Abstract
B-cell activating factor from the tumor necrosis factor family (BAFF) has revealed its critical role in B cell proliferation and survival, as well as the pathogenesis of T-cell mediated autoimmune disease. However, the effect and molecular mechanisms of BAFF on T cell physiological function have not been fully elucidated. In this study it was seen that BAFF can promote the vitality of purified T cells, increase the proportion of CD3 + CD4 + , CD4 + CD25 + , CD4 + CD154 + , and CD4 + CD69 + subgroups and reduce the proportion of CD4 + CD62L + subgroups. Negating BAFF activity with Atacicept (TACI-Fc) reverses vitality and activation of T cells. Furthermore, immunofluorescence detection revealed that BAFF promotes the expression of BAFF receptor (BAFF-R) and transmembrane activator and CAML interactor (TACI) in T cells. Flow cytometry displayed that BAFF/BAFF-R activates the PI3K-Akt signaling pathway while the application of PI3K inhibitor (wortmannin) illuminated that BAFF induces T cell vitality and activation through the PI3K-Akt signaling pathway. We conclude that BAFF is involved in not only the physiology of B cells, but also that of T cells. BAFF affects physiological T-cell activation through BAFF-R-mediated activation of the PI3K-Akt signaling pathway which mirrors one of the pathological mechanisms of T cell-mediated autoimmune diseases.
Original language | English (US) |
---|---|
Article number | 108796 |
Journal | Biomedicine and Pharmacotherapy |
Volume | 114 |
DOIs | |
State | Published - Jun 1 2019 |
Externally published | Yes |
Keywords
- B-cell activating factor from the tumor necrosis factor family (BAFF)
- B-cell activating factor from the tumor necrosis factor family receptor (BAFF-R)
- Phosphoinositide 3-kinase (PI3K)
- T cell activation
- Transmembrane activator and CAML interactor (TACI)
ASJC Scopus subject areas
- Pharmacology